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Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2

Journal of Molecular Neuroscience volume 56, pages 999–1008 (2015)Cite this article

Abstract

Our previous study has showed that co-grafted Schwann cells (SCs) promote proliferation and migration of the grafted oligodendrocyte precursor cells (OPCs). However, how the co-grafted SCs affect OPCs has not been clarified. In the present study, we confirmed that SC-induced proliferation and migration of OPCs were mediated by SC-secreted factors using SC-conditioned medium (SCM). Then, we detected several candidate factors, PDGF-AA, FGF-2, and IGF-1, in SCs and SCM, and their receptors in OPCs. Finally, by using the selective inhibitors, the effects of these candidate factors on proliferation and migration of OPCs were examined. Our results showed that SCM-stimulated proliferation and migration of OPCs could be markedly decreased by both AG1295 (the inhibitor of PDGFR) and PD173074 (the inhibitor of FGFR). Together, our study suggests that SCs affect proliferation and migration of OPCs through secreting PDGF-AA and FGF-2. Identity of these molecules not only contributes to understand the mechanism of SC-induced proliferation and migration of OPCs but also provides possible target for treatment of CNS diseases.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (nos. 81171465 and 81471277).

Conflict of Interest

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    Affiliations

    1. Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People’s Republic of China

      Yue-Juan Chen, Jing-Xing Zhang, Zheng-Rong Zhong & Jian-Guo Hu

    2. Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People’s Republic of China

      Yue-Juan Chen, Jing-Xing Zhang, Lin Shen, Qi Qi, Zheng-Rong Zhong, Zhi-Quan Jiang, Rui Wang, He-Zuo Lü & Jian-Guo Hu

    3. Laboratory of Cell Biology and Molecular Genetics, College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, People’s Republic of China

      Xiao-Xin Cheng

    4. Central Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People’s Republic of China

      He-Zuo Lü

    Authors
    1. Yue-Juan Chen
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    2. Jing-Xing Zhang
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    4. Qi Qi
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    Correspondence to He-Zuo Lü or Jian-Guo Hu.

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    Yue-Juan Chen and Jing-Xing Zhang contributed equally to this work.

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    Chen, YJ., Zhang, JX., Shen, L. et al. Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2. J Mol Neurosci 56, 999–1008 (2015). https://doi.org/10.1007/s12031-015-0570-1

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    • DOI: https://doi.org/10.1007/s12031-015-0570-1

    Keywords

    • Schwann cells
    • Oligodendrocyte progenitor cells
    • Proliferation
    • Migration
    • PDGF-AA
    • FGF-2