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Comparative Proteomic Approach in Rat Model of Absence Epilepsy

Abstract

The aim of this study was to investigate cellular proteins in the pathogenesis of the genetic rat model of absence epilepsy. Protein spots were identified with peptide mass fingerprinting analysis using matrix-assisted laser desorption ionization time of flight mass spectrometry. Data were gathered from the frontoparietal cortex and thalamus of Wistar Albino Glaxo/Rij (WAG/Rij) and Wistar by using two-dimensional gel electrophoresis (2D-PAGE). Six proteins (Clathrin light chain-A protein, Transmembrane EMP24 Domain-Containing Protein, Stathmin-4, Myosin Light Chain4, Rheb, phosphoserine phosphatase) were found to be differentially expressed in the frontoparietal cortex of WAG/Rij and Wistar rats in both age groups. Another set of six proteins (Protein FAM89A and Oasl1, Gemin2, NuDEL1, Pur-beta, 3-alpha HSD) were found to be differentially expressed in the thalamus of WAG/Rij and Wistar rats. Findings from the frontoparietal cortex suggest the presence of altered serine metabolism and increased vesicular trafficking in the frontoparietal cortex of WAG/Rij rats compared with Wistar rats. These differences in the protein levels might reflect the crucial role of these proteins and related pathways in the generation of absence seizures. In the thalamic specimens, age-dependent changes in protein expression were remarkable, suggesting that this phenomenon may be a precursor or a consequence of absence seizures. Our findings further highlight the potential role of the mTOR signaling pathway in absence epilepsy.

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Acknowledgments

This study was supported by TÜBİTAK number 108S196 granted to Ayşe Karson. We would like to thank Dr. Fuat Balcı for his valuable input into the earlier drafts of this manuscript. We confirm that we have read the journal’s position on issues involved in ethical publication and affirm that this report is consistent with those guidelines.

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None of the authors have any conflict of interest to disclose.

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Gürol, G., Demiralp, D.Ö., Yılmaz, A.K. et al. Comparative Proteomic Approach in Rat Model of Absence Epilepsy. J Mol Neurosci 55, 632–643 (2015). https://doi.org/10.1007/s12031-014-0402-8

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  • DOI: https://doi.org/10.1007/s12031-014-0402-8

Keywords

  • WAG/Rij
  • Rheb
  • mTOR
  • 3α-HSD
  • RB3