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Effect of the PPARγ C161T Gene Variant on Serum Lipids in Ischemic Stroke Patients with and Without Type 2 Diabetes Mellitus

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Abstract

Peroxisome proliferator-activated receptor γ (PPARγ) is a ligand-activated transcription factor involved in the regulation of lipid metabolism, diabetes, obesity, atherogenesis and inflammation. PPARγ genetic variation has been associated with metabolic and cardiovascular diseases. The aim of this study was to explore, for the first time, the relationship between PPARγ C161T polymorphism and the risk of ischemic stroke (IS) among patients with type 2 diabetes mellitus (T2DM). A total of 196 patients with IS (117 diabetics and 79 nondiabetics) and 192 controls were recruited to enroll in this study. PPARγ C161T genotyping was performed by PCR-RFLP technique. The 161T allele as compared with C allele was found to be higher in controls than in IS patients (with or without T2DM). After adjusting for multiple risk factors, the T allele carriers had significantly reduced IS risk (OR = 0.575, 95 % CI 0.348–0.951, p = 0.030) compared to the CC homozygotes which increased significantly the risk in IS patients with T2DM (OR = 1.85, 95 % CI 1.23–2.62). Moreover, the triglycerides (TG) and ApoB levels in CC homozygote carriers were significantly higher than those in T allele carriers. These results indicate that the C161T of PPARγ may reduce the risk of IS by modulation of adipose metabolism especially TG and ApoB in IS patients with T2DM.

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Acknowledgments

We would like to thank Dr. Nouira Samir of the Emergency Department at Fattouma Bourguiba University Hospital in Monastir (Tunisia) for recruiting stroke patients and for his help and Dr. Mahdouani Kacem of Molecular biology Laboratory, Ibn Eljazzar Hospital, Kairouan (Tunisia) for the measurements of lipids and lipoproteins and for his help. Also we would like to thank Professor Moncef Rassas for his assistance with the English revision. A part of this work was supported by a grant from “The Tunisian Ministry of Higher Education, Scientific Research and Information and Communication Technologies”.

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Authors and Affiliations

  1. Research Unit: UR 12ES09 Dyslipidemia and Atherogenesis, Faculty of Medicine, Monastir, 5019, Tunisia

    Khouloud Chehaibi & Mohamed Naceur Slimane

  2. Emergency Department, CHU Fattouma Bourguiba, Monastir, 5000, Tunisia

    Samir Nouira

  3. Molecular Biology Laboratory, Ibn Eljazzar Hospital, Kairouan, 3140, Tunisia

    Kacem Mahdouani

  4. Department of Cardiovascular Diseases, Fattouma Bourguiba Hospital, Monastir, 5000, Tunisia

    Sonia Hamdi

  5. UMR 7079 Physiology and Physiopathology, University Pierre and Marie Curie, Paris 6, France

    Mustapha Rouis

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  1. Khouloud Chehaibi
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Chehaibi, K., Nouira, S., Mahdouani, K. et al. Effect of the PPARγ C161T Gene Variant on Serum Lipids in Ischemic Stroke Patients with and Without Type 2 Diabetes Mellitus. J Mol Neurosci 54, 730–738 (2014). https://doi.org/10.1007/s12031-014-0326-3

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