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Upregulation of EHD2 after Intracerebral Hemorrhage in Adult Rats

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Abstract

EHD2, a member of the Eps15 homology domain (EH domain) family, is important for protein interactions during vesicular trafficking. Previous studies have proved that EHD2 can regulate trafficking from the plasma membrane in the process of endocytosis. However, its function in central nervous system diseases is still with limited understanding. In this frame, we found that EHD2 expression was upregulated in the perihematomal caudate in adult rats after intracerebral hemorrhage (ICH). Double immunofluorescence staining revealed that EHD2 was colocalized with neurons and activated microglias after ICH. Besides, we detected that neuronal apoptosis markers (TUNEL and caspase-3), and microglial activation marker (CD68), also known as a marker of macrophage, were colocated with EHD2. The vitro study also indicated that EHD2 was linked with neuronal apoptosis and microglial phagocytosis. All our findings suggested that EHD2 might be involved in the pathophysiology of ICH.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81371299).

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Authors and Affiliations

  1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People’s Republic of China

    Kaifu Ke, Ying Rui, Lei Li, Heyi Zheng, Wei Xu, Xiang Tan, Jianhua Cao, Xiaoyan Wu, Gang Cui & Maohong Cao

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  1. Kaifu Ke
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  2. Ying Rui
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  3. Lei Li
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  4. Heyi Zheng
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  5. Wei Xu
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  6. Xiang Tan
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  7. Jianhua Cao
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  8. Xiaoyan Wu
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  9. Gang Cui
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  10. Maohong Cao
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Corresponding author

Correspondence to Maohong Cao.

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Kaifu Ke and Ying Rui contributed equally to this work.

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Ke, K., Rui, Y., Li, L. et al. Upregulation of EHD2 after Intracerebral Hemorrhage in Adult Rats. J Mol Neurosci 54, 171–180 (2014). https://doi.org/10.1007/s12031-014-0271-1

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  • DOI: https://doi.org/10.1007/s12031-014-0271-1

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