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Differential Regulation of Lipid Metabolism Genes in the Brain of Acetylcholinesterase Knockout Mice

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Abstract

Mice deficient in acetylcholinesterase (AChE; EC3.1.1.7) exhibited significant phenotypical and biochemical changes when compared with wild-type littermates. They showed a delay of growth in weight and size, immature external ears, and persistent body tremor, and they circled when walking. The molecular mechanisms underlying these changes have not been investigated yet. Here, we studied the profiles of both the messenger RNA (mRNA) and protein expression in the brain of AChE-deficient mice using mRNA microarray, quantitative PCR, and two-dimensional difference gel electrophoresis (2D DIGE) coupled to protein identification with matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry. Analysis of gene expression profile was conducted by DAVID (http://david.abcc.ncifcrf.gov) and Ingenuity Pathway Analysis (IPA, http://www.ingenuity.com). Previous results implicated that there is a close relationship between lipid metabolisms which were associated with central nervous system development. Here, we demonstrated that the mRNA expressions of brain specific fatty acid protein 7 (fabp-7) and phospholipase A2 group IV (pla2g4) were significantly downregulated in AChE-deficient mice. These results suggested that AChE may play a role in neurogenesis and neurodegeneration by specifically regulating lipid metabolism in the brain.

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Authors and Affiliations

  1. School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, SAR, China

    Huang-Quan Lin & Chi-Cheong David Wan

  2. Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China

    Huang-Quan Lin

  3. The Chinese University of Hong Kong, 326, Lo Kwee-Seong Building School of Biomedical Sciences Hong Kong, Hong Kong, SAR, China

    Yan Wang

  4. Institute of Integrative Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China

    Kam-Leung Chan

  5. School of Biomedical Sciences, The Chinese University of Hong Kong, 326 LISBS, Area 39, Hong Kong, SAR, China

    Tsz-Ming Ip

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  1. Huang-Quan Lin
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  2. Yan Wang
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  3. Kam-Leung Chan
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  4. Tsz-Ming Ip
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  5. Chi-Cheong David Wan
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Correspondence to Chi-Cheong David Wan.

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Lin, HQ., Wang, Y., Chan, KL. et al. Differential Regulation of Lipid Metabolism Genes in the Brain of Acetylcholinesterase Knockout Mice. J Mol Neurosci 53, 397–408 (2014). https://doi.org/10.1007/s12031-014-0267-x

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  • DOI: https://doi.org/10.1007/s12031-014-0267-x

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