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Journal of Molecular Neuroscience

, Volume 50, Issue 2, pp 291–297 | Cite as

Expression Patterns of miR-124, miR-134, miR-132, and miR-21 in an Immature Rat Model and Children with Mesial Temporal Lobe Epilepsy

  • Jing Peng
  • Ahmed Omran
  • Muhammad Usman Ashhab
  • Huimin Kong
  • Na Gan
  • Fang He
  • Fei YinEmail author
Article

Abstract

Mesial temporal lobe epilepsy (MTLE) is a particularly devastating form of human epilepsy with significant incidence of medical intractability. MicroRNAs (miRs) are small, noncoding RNAs that regulate the posttranscriptional expression of protein-coding mRNAs, which may have key roles in the pathogenesis of MTLE development. To study the dynamic expression patterns of brain-specific miR-124 and miR-134 and inflammation-related miR-132 and miR-21, we performed qPCR on the hippocampi of immature rats at 25 days of age. Expressions were monitored in the three stages of MTEL and in the control hippocampal tissues corresponding to the same timeframes. A similar expression method was applied to hippocampi obtained from children with MTLE and normal controls. The expression patterns of miR-124 and miR-134 nearly showed the same dynamics in the three stages of MTLE development. On the other hand, miR-132 and miR-21 showed significant upregulation in acute and chronic stages, while in the latent stage, miR-132 was upregulated and miR-21 was downregulated. The four miRs were upregulated in hippocampal tissues obtained from children with MTLE. The significant upregulation of miR-124 and miR-134 in the seizure-related stages and children suggested that both can be potential targets for anticonvulsant drugs in the epileptic developing brains, while the different expression patterns of miR-132 and miR-21 may suggest different functions in MTLE pathogenesis.

Keywords

Mesial temporal lobe epilepsy miR-124 miR-134 miR-132 miR-21 Developing brains 

Notes

Acknowledgments

This work was kindly supported by the National Natural Science Foundation of China (nos. 30872790, 30901631, 81171226, 81100846) and the Scientific and Technological Department of Hunan Province (2011FJ3163).

Conflict of interest

None of the authors have any conflict of interest to disclose.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Jing Peng
    • 1
  • Ahmed Omran
    • 1
    • 2
  • Muhammad Usman Ashhab
    • 1
  • Huimin Kong
    • 1
  • Na Gan
    • 1
  • Fang He
    • 1
  • Fei Yin
    • 1
    Email author
  1. 1.Department of PediatricsXiangya Hospital of Central South UniversityChangshaChina
  2. 2.Department of Pediatrics and NeonatologySuez Canal UniversityIsmailiaEgypt

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