Abstract
The genetic manipulation of the laboratory mouse has been well developed and generated more and more mouse lines for biomedical research. To advance our science exploration, it is necessary to share genetically modified mouse lines with collaborators between institutions, even in different countries. The transfer process is complicated. Significant paperwork and coordination are required, concerning animal welfare, intellectual property rights, colony health status, and biohazard. Here, we provide a practical example of importing a transgenic mice line, Dynamin 1 knockout mice, from Yale University in the USA to Perking University in China for studying cell secretion. This example including the length of time that required for paper work, mice quarantine at the receiving institution, and expansion of the mouse line for experiments. The procedure described in this paper for delivery live transgenic mice from USA to China may serve a simple reference for transferring mouse lines between other countries too.
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Acknowledgements
We thank Dr. I.C. Bruce for helpful comments on the manuscript. We thank staffs of the Laboratory Animal Center of Peking University and Beijing Sun-rising Technologies Co. for their excellent services. This work was supported by grants from the National Basic Research Program of China (2007CB512100, 2012CB518006, and 2006CB500800), the National Natural Science Foundation of China (30728009, 30770521, 30770788, 30970660, 30911120491, 30830043, and 31330024).
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Kuo ST, Wu QH, and Liu B are equal contributors.
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Kuo, S.T., Wu, Q.H., Liu, B. et al. Importing, Caring, Breeding, Genotyping, and Phenotyping a Genetic Mouse in a Chinese University. J Mol Neurosci 53, 487–492 (2014). https://doi.org/10.1007/s12031-013-0195-1
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DOI: https://doi.org/10.1007/s12031-013-0195-1