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Localization and Chemical Coding of the Dorsal Motor Vagal Nucleus (DMX) Neurons Projecting to the Porcine Stomach Prepyloric Area in the Physiological State and After Stomach Partial Resection

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Abstract

The aim of our study was to localize and define immunocytochemical characteristic of the dorsal motor nucleus of the vagus (DMX) neurons projecting to the porcine stomach prepyloric region in the physiological state and after gastric partial resection. To identify the stomach-projecting perikarya, the neuronal retrograde tracer—Fast Blue (FB) was injected into the studied region of control and resection group (RES). In the RES group, on 22nd day after FB injection, the partial resection of the stomach region previously injected with FB was performed. Sections were immunostained with ChAT, pituitary adenylate cyclase-activating peptide (PACAP), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), galanin (GAL), substance P (SP), leu-enkephalin (LENK), and cocaine- and amphetamine-regulated transcript (CART). In the DMX of control and RES group, the stomach-projecting perikarya were found in the entire extent of the nucleus bilaterally. Within control animals, 30.08 ± 1.97 % of the gastric DMX perikarya expressed PACAP, while other substances were found only in the neuronal fibers. In the RES group DMX, PACAP was found in 45.58 ± 2.2 %, VIP in 28.83 ± 3.63 %, NOS in 21.22 ± 3.32 %, and GAL in 5.67 ± 1.49 % of the FB-labeled gastric perikarya. Our data implicate PACAP, VIP, NOS, and GAL as neuronal survival promoting substances and the CART-, LENK-, SP- NOS-, and GAL-immunoreactive processes in control of the gastric vagal neurons in the pig.

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Acknowledgments

This study was supported by the Polish State Committee for Scientific Research number 1890/B/P01/2010/39.

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  1. Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718, Olsztyn, Poland

    Marta Gańko & Jarosław Całka

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  1. Marta Gańko
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  2. Jarosław Całka
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Correspondence to Marta Gańko.

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Gańko, M., Całka, J. Localization and Chemical Coding of the Dorsal Motor Vagal Nucleus (DMX) Neurons Projecting to the Porcine Stomach Prepyloric Area in the Physiological State and After Stomach Partial Resection. J Mol Neurosci 52, 90–100 (2014). https://doi.org/10.1007/s12031-013-0102-9

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