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Upregulation of Sestrin-2 Expression via P53 Protects Against 1-Methyl-4-Phenylpyridinium (MPP+) Neurotoxicity

Journal of Molecular Neuroscience volume 51, pages 967–975 (2013)Cite this article

Abstract

Sestrin-2 (SESN2) is a conserved antioxidant protein that is activated upon oxidative stress and protects cells against reactive oxygen species (ROS). However, the role of SESN2 in neurodegenerative diseases, especially in Parkinson’s disease (PD), has not yet been elucidated. In this study, we found that expression of SESN2 is elevated in the midbrain of patients with PD. Moreover, in vitro experiments display that the drug 1-methyl-4-phenylpyridinium (MPP+) induces the expression of SESN2 in SH-SY5Y cells in a time- and dose-dependent manner. Our results show that p53 is activated by MPP+. Importantly, inhibition of p53 using small RNA interferences abolishes the increased SESN2 levels induced by MPP+, suggesting that the inductive effect of MPP+ on SESN2 is mediated by p53. Furthermore, knockdown of SESN2 using small RNA interferences promotes MPP+-related neurotoxicity by attenuating oxidative stress, mitochondrial dysfunction, and apoptosis. All these data imply that the induction of SESN2 produces a protective effect in PD.

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Affiliations

  1. Intensive Care Unit of Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jiefang Avenue, Wuhan, 430030, Hubei, China

    Zhou Daixing, Zhan Chengye, Zhong Qiang & Li Shusheng

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  1. Zhou Daixing
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  2. Zhan Chengye
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  3. Zhong Qiang
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  4. Li Shusheng
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Correspondence to Zhan Chengye.

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Daixing, Z., Chengye, Z., Qiang, Z. et al. Upregulation of Sestrin-2 Expression via P53 Protects Against 1-Methyl-4-Phenylpyridinium (MPP+) Neurotoxicity. J Mol Neurosci 51, 967–975 (2013). https://doi.org/10.1007/s12031-013-0081-x

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  • DOI: https://doi.org/10.1007/s12031-013-0081-x

Keywords

  • Parkinson’s disease
  • Sestrin-2 (SESN2)
  • p53
  • Oxidative stress
  • Mitochondrial dysfunction
  • Apoptosis