Abstract
Transcription initiation factor IIB (TFIIB) is an ideal factor to localize core promoters and plays a central role in the assembly of the pre-initiation complex. Previous studies showed that the assembly of TFIIB played an important role in rat ischemic brain injury. To elucidate the expression and possible functions of TFIIB in retina lesion and repair, we performed an optic nerve crush (ONC) model in adult rats. Western blot analysis and immunohistochemistry showed a significant upregulation of TFIIB in retina after ONC. Immunofluorescent labeling indicated that TFIIB was localized mainly in the Müller glia cells (MGCs); colocalization of TFIIB and proliferating cell nuclear antigen (PCNA) in the injured retina suggested that TFIIB might participate in MGCs proliferation. In addition, we also examined the expression of the retinal progenitor markers (Nestin and Pax6) whose changes were correlated with the expression of TFIIB. In vitro, we induced MGCs differentiation with brain nerve growth factor (BNGF) and found that TFIIB expression was increased in the differentiated process, which was collected with the expression of PCNA, Nestin, and Pax6. Additionally, knocking TFIIB down with siRNA inhibited the expression of PCNA, Nestin, and Pax6. Collectively, we hypothesized ONC-induced upregulation of TFIIB in the retina was associated with MGCs activation and differentiation.
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Acknowledgments
The authors would like to thank Dr. Zhifeng Gu for technical assistance. This work was supported by Health Department Research Projects in Jiangsu Province (grant no. Z200807), Science and Technology Projects in Nantong (grant no. S2010020), Graduate Student Innovation of Science and Technology Projects in Jiangsu Province, and Graduate Student Innovation of Science and Technology Projects in Nantong University (grant no. 13025045).
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Yue Xu and Chen Chen contributed equally to this work.
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Xu, Y., Chen, C., Jin, N. et al. Müller Glia Cells Activation in Rat Retina After Optic Nerve Injury: Spatiotemporal Correlation with Transcription Initiation Factor IIB. J Mol Neurosci 51, 37–46 (2013). https://doi.org/10.1007/s12031-012-9941-z
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DOI: https://doi.org/10.1007/s12031-012-9941-z