The Combined Effect of Sleep Deprivation and Western Diet on Spatial Learning and Memory: Role of BDNF and Oxidative Stress
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Either sleep deprivation or Western diet can impair learning and memory via induction of oxidative stress, which results in neuronal damage and interference with the neurotransmission. In this study, we examined the combined effect of sleep deprivation and Western diet on hippocampus-dependent spatial learning and memory. In addition, possible molecular targets for sleep deprivation and Western diet-induced cognitive impairments were investigated. Sleep deprivation was induced in rats using the modified multiple platform model simultaneous with the administration of Western diet for 6 weeks. Thereafter, spatial learning and memory were tested using radial arm water maze. At the molecular level, BDNF protein and antioxidant markers including superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG, and thiobarbituric acid reactive substances (TBARS) were assessed. The results of this study revealed that sleep deprivation, Western diet, or a combination of both impair short- and long-term memory (P < 0.05). The magnitude of the impairment induced by the combined treatment at the 24-h long-term memory was higher than that caused by each factor alone (P < 0.05). In addition, the combined treatment reduced the levels of hippocampal BDNF, a reduction that was not detected with each factor alone. Moreover, the combined treatment reduced the hippocampal activities of SOD, catalase, GPx, ratio of GSH/GSSG, and elevated TBARS level (P < 0.05). In conclusion, the combination of sleep deprivation and Western diet decreases BDNF levels and increases oxidative stress in the hippocampus, thus inducing memory impairment that is greater than the impairment produced by each factor alone.
KeywordsSleep deprivation Western diet Maze Memory BDNF Oxidative stress Learning Hippocampus
This project was supported by grants (193/2009, 213/2010, 146/2011) from the Deanship of Research at the Jordan University of Science and Technology.
- Garcia-Fuentes E, Murri M, Garrido-Sanchez L, Garcia-Serrano S, Garcia-Almeida JM, Moreno-Santos I, Tinahones FJ, Macias-Gonzalez M (2010) PPARgamma expression after a high-fat meal is associated with plasma superoxide dismutase activity in morbidly obese persons. Obesity (Silver Spring) 18:952–958CrossRefGoogle Scholar
- Guyton AC, Hall JE (2006) States of brain activity—sleep, brain waves, epilepsy, phycosis sleep: Medical physiology, 11th edn. Elsevier, Philadelphia, pp 739–741Google Scholar
- Harikesh Kalonia AK (2006) Protective effect of BR-16A (poly herbal preparation) on the behavioural and biochemical alterations in sleep disturbed mice. Annals of Neurosciences 13Google Scholar
- Khabour OF, Alzoubi KH, Alomari MA, Alzubi MA (2012) Changes in spatial memory and BDNF expression to simultaneous dietary restriction and forced exercise. Brain Res Bull. doi: 10.1016/j.brainresbull.2012.08.005
- Silva RH, Abilio VC, Takatsu AL, Kameda SR, Grassl C, Chehin AB, Medrano WA, Calzavara MB, Registro S, Andersen ML, Machado RB, Carvalho RC, Ribeiro Rde A, Tufik S, Frussa-Filho R (2004) Role of hippocampal oxidative stress in memory deficits induced by sleep deprivation in mice. Neuropharmacology 46:895–903PubMedCrossRefGoogle Scholar
- White CL, Pistell PJ, Purpera MN, Gupta S, Fernandez-Kim SO, Hise TL, Keller JN, Ingram DK, Morrison CD, Bruce-Keller AJ (2009) Effects of high fat diet on Morris maze performance, oxidative stress, and inflammation in rats: contributions of maternal diet. Neurobiol Dis 35:3–13PubMedCrossRefGoogle Scholar