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Upregulation of Astrocytic Leptin Receptor in Mice with Experimental Autoimmune Encephalomyelitis

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Abstract

The detrimental role of leptin in experimental autoimmune encephalomyelitis (EAE) is opposite to its neuroprotective role in other neuropathologies. We hypothesize that a shifted cellular distribution of leptin receptors underlies the differential effects of leptin. A robust increase of ObR immunoreactivity was seen along glial fibrillary acidic protein (GFAP)(+) intermediate filaments in reactive astrocytes in the hippocampus and hypothalamus of mice with EAE. Although astrocyte-specific GFAP mRNA and protein were both increased, ObRa mRNA was elevated only after resolution of EAE symptoms, and ObRb mRNA was even decreased at the peak time of symptoms of EAE. A cell type-specific action of leptin may underlie its differential effects.

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Acknowledgments

This study was supported by NIH (NS62291 to WP and DK54880 and DK92245 to AJK). We thank Dr. David Burk from the Cellular Imaging Core Facility of PBRC for the helpful suggestions on image quantification.

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  1. Xiaojun Wu

    Present address: Shanghai University of Traditional Chinese Medicine, Shanghai, China

Authors and Affiliations

  1. Blood-Brain Barrier Group, Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA, 70808, USA

    Xiaojun Wu, Hung Hsuchou, Abba J. Kastin, Pramod K. Mishra & Weihong Pan

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Correspondence to Weihong Pan.

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Wu, X., Hsuchou, H., Kastin, A.J. et al. Upregulation of Astrocytic Leptin Receptor in Mice with Experimental Autoimmune Encephalomyelitis. J Mol Neurosci 49, 446–456 (2013). https://doi.org/10.1007/s12031-012-9825-2

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  • DOI: https://doi.org/10.1007/s12031-012-9825-2

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