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Cortical 5HT2A Receptor Function under Hypoxia in Neonatal Rats: Role of Glucose, Oxygen, and Epinephrine Resuscitation

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Abstract

Neonatal hypoxia induces brain injury through alterations in neurotransmitters and its receptors. Molecular processes regulating serotonergic receptors play an important role in the control of respiration under hypoxia. The present study evaluates the serotonergic regulation of neonatal hypoxia and its resuscitation methods. Receptor binding assays and gene expression studies were done to evaluate the changes in 5HT2A receptors and its transporter in the cerebral cortex of hypoxic neonatal rats and hypoxic rats resuscitated with glucose, oxygen, and epinephrine. Hypoxic stress increased total 5HT and 5HT2A receptor number along with an upregulation of 5HT2A receptor and 5HT transporter gene in the cortex. The enhanced cortical 5HT2A receptors may act as a modulator of ventilatory response to hypoxia. These alterations were reversed to near control by glucose supplementation. Glucose supplementation helped in managing the serotonergic functional alterations. Hypoxia-induced adenosine triphosphate depletion causes a reduction in blood glucose levels which can be encountered by glucose administration, and oxygenation helps in overcoming the anaerobic condition. The adverse effect of immediate oxygenation and epinephrine supplementation was also reported. This has immense clinical significance in establishing a proper resuscitation for the management of neonatal hypoxia.

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Acknowledgments

This work was supported by the research grants from DBT, DST, ICMR, government of India and KSCSTE, government of Kerala to Dr. C. S. Paulose. T.R. Anju thanks the Council of Scientific and Industrial Research for the Senior Research Fellowship.

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Authors and Affiliations

  1. Molecular Neurobiology and Cell Biology Unit, Center for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin, 682022, Kerala, India

    T. R. Anju, S. Smijin, P. K. Korah & C. S. Paulose

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  1. T. R. Anju
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  2. S. Smijin
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  3. P. K. Korah
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  4. C. S. Paulose
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Correspondence to C. S. Paulose.

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Anju, T.R., Smijin, S., Korah, P.K. et al. Cortical 5HT2A Receptor Function under Hypoxia in Neonatal Rats: Role of Glucose, Oxygen, and Epinephrine Resuscitation. J Mol Neurosci 43, 350–357 (2011). https://doi.org/10.1007/s12031-010-9449-3

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  • DOI: https://doi.org/10.1007/s12031-010-9449-3

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