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Expression Profile of Lgi1 Gene in Mouse Brain During Development

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Abstract

Mutations in LGI1 were described in patients with autosomal dominant partial epilepsy with auditory features (ADPEAF), and recent clinical findings have implicated LGI1 in human brain development. However, the precise role of LGI1 in epileptogenesis remains largely unknown. The objective of this study was to determine the expression pattern of Lgi1 in mice brain during development and in adult animals. Real-time polymerase chain reaction (PCR) quantification and Western blot experiments showed a relative low expression during intrauterine stages, increasing until adulthood. In addition, we did not find significant differences between left and right hemispheres. The hippocampus presented higher levels of Lgi1 expression when compared to the neocortex and the cerebellum of adult animals; however, these results did not reach statistical significance. This study was the first to determine a specific profile of Lgi1 gene expression during central nervous system development, which suggests a possible inhibitory function in latter stages of development. In addition, we did not find differences in hemispheric expression that could explain the predominance of left-sided abnormalities in patients with ADPEAF.

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References

  • Abramoff, M. D., Magelhaes, P. J., & Ram, S. J. (2004). Image processing with image. Journal in Biophotonics International, 11(7), 36–42.

    Google Scholar 

  • Ayres, M., Ayres Jr., M., Ayres, D. L., & Dos Santos, A. S. (2003). BioEstat 3.0: Aplicações estatísticas nas áreas das Ciências Biológicas e Médicas. Belém: Sociedade civil Mamiruá, Brasília.

    Google Scholar 

  • Brodtkorb, E., Gu, W., Nakken, K. O., Fischer, C., & Steinlein, O. K. (2002). Familial temporal lobe epilepsy with aphasic seizures and linkage to chromosome 10q22-q24. Epilepsia, 43(3), 228–235.

    Article  PubMed  CAS  Google Scholar 

  • Brodtkorb, E., Steinlein, O. K., & Sand, T. (2005). Asymmetry of long-latency auditory evoked potentials in LGI1-related autosomal dominant lateral temporal lobe epilepsy. Epilepsia, 46(10), 1692–1694.

    Article  PubMed  Google Scholar 

  • Cendes, F., Kobayashi, E., & Lopes-Cendes, I. (2005). Familial temporal lobe epilepsy with auditory features. Epilepsia, 46(Suppl. 10), 59–60.

    Article  PubMed  Google Scholar 

  • Chernova, O. B., Somerville, R. P. T., & Cowell, J. K. (1998). A novel gene, LGI1, from 10q24 is rearranged and downregulated in malignant brain tumors. Oncogene, 17, 2873–2881.

    Article  PubMed  CAS  Google Scholar 

  • Deshane, J., Chaves, L., Sarikonda, K. V., et al. (2004). Proteomics analysis of rat brain protein modulations by grape seed extract. Journal of Agricultural and Food Chemistry, 52(26), 7872–7883.

    Article  PubMed  CAS  Google Scholar 

  • Fukata, Y., Adesnik, H., Iwanaga, T., Bredt, D. S., Nicoll, R. A., & Fukata, M. (2006). Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission. Science, 313(5794), 1792–1795.

    Article  PubMed  CAS  Google Scholar 

  • Grosse, G., Draguhn, A., Höhne, L., Tapp, R., Veh, R. W., & Ahnert-Hilger, G. (2000). Expression of Kv1 potassium channels in mouse hippocampal primary cultures: Development and activity-dependent regulation. The Journal of Neuroscience, 20(5), 1869–1882.

    PubMed  CAS  Google Scholar 

  • Gu, W., Brodtkorb, E., Piepoli, T., Finocchiaro, G., & Steinlein, O. K. (2005). LGI1: A gene involved in epileptogenesis and glioma progression. Neurogenetics, 6(2), 59–66.

    Article  PubMed  CAS  Google Scholar 

  • Gu, W., Brodtkorb, E., & Steinlein, O. K. (2002). LGI1 is mutated in familial temporal lobe epilepsy characterized by aphasic seizures. Annals of Neurology, 52, 364–367.

    Article  PubMed  CAS  Google Scholar 

  • Head, K., Gong, S., Joseph, S., et al. (2007). Defining the expression pattern of the LGI1 gene in BAC transgenic mice. Mamm. Genome, 18(5), 328–337.

    CAS  Google Scholar 

  • Kalachikov, S., Evgrafov, O., Ross, B., et al. (2002). Mutations in LGI1 cause autosomal-dominant partial epilepsy with auditory features. Nature Genetics, 30, 335–341.

    Article  PubMed  Google Scholar 

  • Kobayashi, E., Santos, N. F., Torres, F. R., et al. (2003). Magnetic resonance imaging abnormalities in familial temporal lobe epilepsy with auditory auras. Archives of Neurology, 60, 1546–1551.

    Article  PubMed  Google Scholar 

  • Krex, D., Hauses, M., Appelt, H., et al. (2002). Physical and functional characterization of the human LGI1 gene and its possible role in glioma development. Acta Neuropathologica, 103(3), 255–266.

    Article  PubMed  CAS  Google Scholar 

  • Kunapuli, P., Chitta, K. S., & Cowell, J. K. (2003). Suppression of the cell proliferation and invasion phenotypes in glioma cells by the LGI1 gene. Oncogene, 22(26), 3985–3991.

    Article  PubMed  CAS  Google Scholar 

  • Liao, D., Zhang, X., O’Brien, R., Ehlers, M. D., & Huganir, R. (1999). Regulation of morphological postsynaptic silent synapses in developing hippocampal neurons. Nature Neuroscience, 2(1), 37–43.

    Article  PubMed  CAS  Google Scholar 

  • Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the \(2^{{ - \Delta \Delta C_{T} }} \) method. Methods, 25(4), 402–408.

    Article  PubMed  CAS  Google Scholar 

  • Marcondes, F. K., Bianchi, F. J., & Tanno, A. P. (2002). Determination of the estrous cycle phases of rats: Some helpful considerations. Brazilian Journal of Biology, 62(4A), 609–614.

    Article  CAS  Google Scholar 

  • Michelucci, R., Poza, J. J., Sofia, V., et al. (2003). Autosomal dominant lateral temporal epilepsy: Clinical spectrum, new epitempin mutations and genetic heterogeneity in seven European families. Epilepsia, 44(10), 1289–1297.

    Article  PubMed  CAS  Google Scholar 

  • Morante-Redolat, J. M., Gorostidi-Pagola, A., Piquer-Sirerol, S., et al. (2002). Mutations in the LGI1/Epitempin gene on 10q24 cause autosomal dominant lateral temporal epilepsy. Human Molecular Genetics, 11, 1119–1127.

    Article  PubMed  CAS  Google Scholar 

  • Onojafe, F. I. (2005). Vaginal mucous plug and weight gain in mice. Tech talk—The newsletter for laboratory animal science technicians, 10(6), 4.

    Google Scholar 

  • Ottman, R., Risch, N., Hauser, W. A., et al. (1995). Localization of a gene for partial epilepsy to chromosome 10q. Nat. Genet, 10(1), 56–60.

    CAS  Google Scholar 

  • Ottman, R., Winawer, M. R., Kalachikov, S., et al. (2004). LGI1 mutations in autosomal dominant partial epilepsy with auditory features. Neurology, 62, 1120–1126.

    PubMed  CAS  Google Scholar 

  • Piepoli, T., Jakupoglu, C., Gu, W., et al. (2006). Expression studies in gliomas and glial cells do not support a tumor suppressor role for LGI1. Neuro Oncology, 8(2), 96–108.

    Article  PubMed  CAS  Google Scholar 

  • Pisano, T., Marini, C., Brovedani, P., et al. (2005). Abnormal phonologic processing in familial lateral temporal lobe epilepsy due to a new LGI1 mutation. Epilepsia, 46(1), 118–123.

    Article  PubMed  CAS  Google Scholar 

  • Pizzuti, A., Flex, E., Di Bonaventura, C., et al. (2003). Epilepsy with auditory features: A LGI1 gene mutation suggests a loss-of-function mechanism. Annals of Neurology, 53, 396–399.

    Article  PubMed  CAS  Google Scholar 

  • Poza, J. J., Saenz, A., Martinez-Gil, A., et al. (1999). Autosomal dominant lateral temporal epilepsy: Clinical and genetic study of a large Basque pedigree linked to chromosome 10q. Annals of Neurology, 45(2), 182–188.

    Article  PubMed  CAS  Google Scholar 

  • Schulte, U., Thumfart, J. O., Klocker, N., et al. (2006). The epilepsy-linked Lgi1 protein assembles into presynaptic Kv1 channels and inhibits inactivation by Kvbeta1. Neuron, 49(5), 697–706.

    Article  PubMed  CAS  Google Scholar 

  • Senechal, K. R., Thaller, C., & Noebels, J. L. (2005). ADPEAF mutations reduce levels of secreted LGI1, a putative tumor suppressor protein linked to epilepsy. Human Molecular Genetics, 14(12), 1613–1620.

    Article  PubMed  CAS  Google Scholar 

  • Somerville, R. P., Chernova, O., Liu, S., Shoshan, Y., & Cowell, J. K. (2000). Identification of the promoter, genomic structure, and mouse ortholog of LGI1. Mammalian Genome, 11(8), 622–627.

    Article  PubMed  CAS  Google Scholar 

  • Tanic, N., Perovic, M., Mladenovic, A., Ruzdijic, S., & Kanazir, S. (2007). Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus—Evaluation by real time RT-PCR. Journal of Molecular Neuroscience, 32(1), 38–46.

    Article  PubMed  CAS  Google Scholar 

  • SAS Institute Inc. (1999–2001). The SAS System for Windows (Statistical Analysis System), v8.02. Cary, NC, USA: SAS Institute Inc.

    Google Scholar 

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Acknowledgment

We thank Tiago Campos Pereira for the helpful review of this manuscript. This study was supported by the State of São Paulo Research Foundation (FAPESP), São Paulo, Brazil. P.A.O.R. received a studentship from the National Council for Scientific and Technological Development (CNPq), Brazil. F.F.G. received a studentship from the Brazilian Education Ministry—CAPES, São Paulo, Brazil.

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Authors and Affiliations

  1. Department of Medical Genetics, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Patrícia A. O. Ribeiro, Fábio F. Conte & Iscia Lopes-Cendes

  2. Department of Surgery, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Lourenço Sbragia

  3. Laboratory of Animal Quality Control–Multidisciplinary Center of Biological Investigation—(CEMIB), University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Rovilson Gilioli

  4. Department of Physiology and Biophysics, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Francesco Langone

  5. Department of Medical Genetics, FCM-UNICAMP, Tessália Vieira de Camargo, 126, Campinas, São Paulo, 13084-971, Brazil

    Iscia Lopes-Cendes

Authors
  1. Patrícia A. O. Ribeiro
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  2. Lourenço Sbragia
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  3. Rovilson Gilioli
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  4. Francesco Langone
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  5. Fábio F. Conte
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  6. Iscia Lopes-Cendes
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Correspondence to Iscia Lopes-Cendes.

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Ribeiro, P.A.O., Sbragia, L., Gilioli, R. et al. Expression Profile of Lgi1 Gene in Mouse Brain During Development. J Mol Neurosci 35, 323–329 (2008). https://doi.org/10.1007/s12031-008-9096-0

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  • DOI: https://doi.org/10.1007/s12031-008-9096-0

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