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Evaluation of Immunotargeted Gold Nanoshells as Rapid Diagnostic Imaging Agents for HER2-Overexpressing Breast Cancer Cells: A Time-based Analysis

Abstract

Biomedical nanotechnology offers superior potential for diagnostic imaging of malignancy at the microscopic level. In addition to current research focused on dual-imaging and therapeutic applications in vivo, these novel particles may also prove useful for obtaining immediate diagnostic results in vitro at the patient bedside. However, translating the use of nanoparticles for cancer detection to point-of-care applications requires that conditions be optimized such that minimal time is needed for diagnostic results to become available. Thus far, no reports have been published on minimizing the time needed to achieve acceptable optical contrast of cancer cells incubated with nanoparticles. In this study, we demonstrate the use of gold nanoshells targeted to anti-HER2 antibodies that produce sufficient optical contrast with HER2-overexpressing SK-BR-3 breast cancer cells in only 5 min. This work validates the proof of concept that nanoshells targeted to extracellular biomarkers can be used to enhance cancer diagnostic imaging for use in point-of-care applications.

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Acknowledgments

We thank Vengadesan Nammalvar and Adrien Wang for expert technical assistance on nanoshell fabrication. We also thank Nastassja Lewinski for SEM imaging and Christine Wogan for editing assistance. This work was supported by a Department of Defense Congressionally Directed Breast Cancer Research Program Era of Hope Scholar Award to Rebekah Drezek and Tse-Kuan Yu, the Center for Biological and Environmental Nanotechnology (EEC-0118007 and EEC-0647452), the Beckman Foundation, and the John and Ann Doerr Fund for Computational Biomedicine.

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Correspondence to Rebekah A. Drezek.

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T.-K. Yu and R.A. Drezek contributed equally to this work.

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Bickford, L.R., Chang, J., Fu, K. et al. Evaluation of Immunotargeted Gold Nanoshells as Rapid Diagnostic Imaging Agents for HER2-Overexpressing Breast Cancer Cells: A Time-based Analysis. Nanobiotechnol 4, 1–8 (2008). https://doi.org/10.1007/s12030-008-9010-4

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  • DOI: https://doi.org/10.1007/s12030-008-9010-4

Keywords

  • nanoshells
  • point-of-care diagnostics
  • optical imaging
  • cancer diagnosis