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Self-assembled monolayer coating of biological probes to avoid protein adhesion

NanoBiotechnology volume 2, pages 61–65 (2006)Cite this article

Abstract

Probes designed to locally illuminate structures within plant cells are described. The probes studied are etch-sharpened single mode optical fibers, coated with aluminum, similar to probes used for near-field scanning optical microscopy. We find that cellular material adheres to the probes that are not coated with a self-assembled monolayer octadecyltrichlorosilane. The hydrophobic monolayer coating enabled these probes to be inserted into and removed from plant cells with no protein adhesion to the probes. This allows probe reinsertion and it causes less damage to the target cell, greatly facilitating in vivo optical study of cells.

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Authors and Affiliations

  1. Department of Physics, North Carolina State University, 27695-8202, Raleigh, NC

    Greg Nusz & Hans D. Hallen

  2. Department of Botany, North Carolina State University, 27695-8202, Raleigh, NC

    Eva Johannes & Nina Strömgren Allen

Authors
  1. Greg Nusz
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  2. Eva Johannes
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  3. Nina Strömgren Allen
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  4. Hans D. Hallen
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Correspondence to Hans D. Hallen.

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Nusz, G., Johannes, E., Allen, N.S. et al. Self-assembled monolayer coating of biological probes to avoid protein adhesion. Nanobiotechnol 2, 61–65 (2006). https://doi.org/10.1007/s12030-006-0008-5

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  • DOI: https://doi.org/10.1007/s12030-006-0008-5

Key Words

  • Adhesion
  • optical fiber probe
  • self-assembled monolayer
  • in vivo optical studies
  • bio compatible nanoscale localization
  • cell biology