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Changes in the Expression of Long Non-Coding RNA SDMGC and Its Target Gene, TRIM16, in Patients with Gastric Cancer

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Abstract

Purpose

Gastric cancer (GC) has been identified worldwide as one of the most common cancer types with a high mortality rate. LncRNA SDMGC has been recognized as an oncogene with regulatory effects on its target gene, TRIM16, which is believed to play a tumor-suppressing role in various cancers. Both these genes are involved in GC development, tumorigenesis, invasion, and metastasis. The current study is aimed to investigate the association of SDMGC and TRIM16 with GC susceptibility and GC patients’ clinicopathological characteristics.

Methods

A total of 100 GC tissues and their corresponding adjacent non-tumor tissues were sampled. Total RNA was then isolated to measure SDMGC and TRIM16 expression levels using quantitative reverse transcriptase (qRT)-PCR. Statistical analyses including the Mann–Whitney U test and correlation tests were carried out using R v4.5. GraphPad Prism was also used to plot the receiver operating curve (ROC).

Results

The results demonstrated the significant overexpression of lncRNAs SDMGC and downregulation of TRIM16 in GC tissues as compared to their corresponding marginal normal tissue samples (P = 0.005 and P = 0.009, respectively). No association with clinicopathological variables was observed for either SDMGC or TRIM16. Moreover, the results demonstrated a small positive correlation between SDMGC and TRIM16. Evaluation of the diagnostic value of SDMGC and TRIM16 showed poor biomarker potency for these genes.

Conclusion

In conclusion, the results indicated an increase in the expression of SDMGC and a decline in the expression pattern of TRIM16 among the Iranian population. The results indicated a key tumor-accelerative function of SDMGC and a pivotal tumor-suppressing role of TRIM16 in GC patients.

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Availability of Data and Materials

The data are available upon request from the corresponding author.

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Authors and Affiliations

  1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Mina Seifi Inallou, Reza Safaralizadeh, Ali Rajabi, Mohammadali Hosseinpourfeizi & Mehdi Haghi

Authors
  1. Mina Seifi Inallou
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  2. Reza Safaralizadeh
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  3. Ali Rajabi
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  4. Mohammadali Hosseinpourfeizi
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  5. Mehdi Haghi
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Contributions

Reza Safaralizadeh and Mohammadali Hosseinpourfeizi conceived and designed the research. Ali Rajabi and Mina Seifi Inallou have acquired all human specimens and data. Ali Rajabi and Mehdi Haghi carried out the data analysis. Reza Safaralizadeh has assisted in analyzing and interpreting the data. Mina Seifi Inallou and Ali Rajabi wrote the manuscript. Reza Safaralizadeh supervised this project and presented revisions for important intellectual content. The final manuscript has been reviewed by all authors and approved for publication.

Corresponding author

Correspondence to Reza Safaralizadeh.

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Ethics Approval and Consent to Participate

All procedures involving human participants were carried out in complete compliance with the ethical standards of the University of Tabriz research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The Medical Ethics Committee of the University of Tabriz approved the study (code: IR. TABRIZU. REC. 1398.015). All patients signed a written informed consent form.

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The authors declare no competing interests.

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Seifi Inallou, M., Safaralizadeh, R., Rajabi, A. et al. Changes in the Expression of Long Non-Coding RNA SDMGC and Its Target Gene, TRIM16, in Patients with Gastric Cancer. J Gastrointest Canc 54, 44–50 (2023). https://doi.org/10.1007/s12029-021-00791-y

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  • DOI: https://doi.org/10.1007/s12029-021-00791-y

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