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Overexpression of lncRNA DLEU1 in Gastric Cancer Tissues Compared to Adjacent Non-Tumor Tissues

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Abstract

Purpose

Gastric cancer (GC) is caused by environmental factors and genetic changes of protein-coding- and non-coding sequences, which entail short non-coding RNAs (microRNAs) and long non-coding RNAs (lncRNAs). DLEU1 (deleted in lymphocytic leukemia 1), as an effective lncRNA located on chromosome 14.3q 13, modulates the nuclear factor-kB (NF-kB) signaling pathway. This gene usually plays an oncogenic role in the tumorigenesis of multiple types of cancer. The present study examined the expression level of DLEU1 and its association with clinical-pathological characteristics in GC.

Methods

Total RNA of 100 specimens was extracted by TRIzol reagent. After cDNA synthesis, qRT-PCR analysis was performed to measure the expression level of the DLEU1 gene and the obtained data were analyzed by SPSS 16.0.

Results

The relative expression level of DLEU1 significantly increased in tumor specimens compared to the normal tumor margin specimens. The biomarker index of lncRNA DLEU1 was 0.7 in tumor tissues. The observed high expression level of DLEU1 was pertinent to the pathological progressive TNM stage, lymph node metastasis, differentiation degree, patient’s age and lifestyle, and Helicobacter pylori infection in GC patients.

Conclusion

The obtained findings suggested that DLEU1 acts as an oncogene in GC and might be a new target for gene therapy of GC.

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Availability of Data and Material

The data were available if requested by the editor and reviewers.

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Acknowledgements

The authors would like to thank the staff of Immunology Research Center, Tabriz University of Medical Sciences, for their assistance throughout this study.

Author information

Authors and Affiliations

  1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Roghieh Ghodrati, Reza Safaralizadeh & Mohammadali Hosseinpourfeizi

  2. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Milad Asadi & Behzad Baradaran

  3. Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Masoud Shirmohammadi

  4. Department of Biology, University College of Nabi Akram, Tabriz, Iran

    Narges Dastmalchi

Authors
  1. Roghieh Ghodrati
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  2. Reza Safaralizadeh
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  3. Narges Dastmalchi
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  4. Mohammadali Hosseinpourfeizi
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  5. Milad Asadi
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  6. Masoud Shirmohammadi
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Corresponding author

Correspondence to Reza Safaralizadeh.

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Ethics Approval and Consent to Participate

The present study was approved by the Ethics Committee of Tabriz University of Medical Sciences, Tabriz, Iran (approval number: IR.TBZMED.REC.1397.638, date: 5 November 2018). All patients signed the written informed consent form. All participants signed the informed consent.

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The authors declare no competing interests.

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Ghodrati, R., Safaralizadeh, R., Dastmalchi, N. et al. Overexpression of lncRNA DLEU1 in Gastric Cancer Tissues Compared to Adjacent Non-Tumor Tissues. J Gastrointest Canc 53, 990–994 (2022). https://doi.org/10.1007/s12029-021-00733-8

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  • DOI: https://doi.org/10.1007/s12029-021-00733-8

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