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The Promising Effect of Peucedanum chenur Chloroformic Extract on Prevention of Human Colorectal Cancer Progression by Modulating miR-135b, miR-21, and APC Genes

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Abstract

Purpose

The therapeutic use of herbal medicines for the diseases, including cancer, is increasing due to their lower side effects. The present research evaluated the effect of Peucedanum chenur chloroformic extract (PCCE) on cell proliferation against HCT-116 human colorectal cancer cell line.

Methods

The cytotoxic effect of PCCE was evaluated by MTT assay. The activity of the Wnt/B-catenin pathway was assayed through measuring the expression of miR-135b, miR-21, and APC genes by real-time PCR. The flow cytometry and scratch tests were used to study the cell cycle and cell migration, respectively. Also, the antioxidant activity of PCCE was measured by DPPH and iron-chelating tests.

Results

The results showed the downregulation of miR-135b and miR-21 and overexpression of the APC gene. Furthermore, PCCE decreased the free radicals, cell migration, and cell proliferation. The antioxidant activity of PCCE was confirmed by standard tests.

Conclusion

Altogether, our findings suggest that purified compounds of PCCE could be developed as a potent chemo-preventive drug for the treatment of CRC.

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Acknowledgements

Thanks are due to Saeed Yadegari for assistance with some experiments.

Funding

This study was funded by the Vice Chancellery for Research and Technology, Hamadan University of Medical Sciences (grant number: 9709135421).

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Authors and Affiliations

  1. Department of Molecular Medicine & Genetics, Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Milad Fadaei & Hamid Eslami

  2. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Razieh Amini & Ali Mahdavinezhad

  3. Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

    Dara Dastan

  4. Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran

    Dara Dastan

Authors
  1. Milad Fadaei
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  2. Razieh Amini
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  3. Dara Dastan
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  4. Hamid Eslami
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  5. Ali Mahdavinezhad
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All the authors contributed in this investigation.

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Correspondence to Ali Mahdavinezhad.

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Fadaei, M., Amini, R., Dastan, D. et al. The Promising Effect of Peucedanum chenur Chloroformic Extract on Prevention of Human Colorectal Cancer Progression by Modulating miR-135b, miR-21, and APC Genes. J Gastrointest Canc 53, 549–556 (2022). https://doi.org/10.1007/s12029-021-00660-8

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  • DOI: https://doi.org/10.1007/s12029-021-00660-8

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