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Assessment of oncogenic role of intestinal microbiota in colorectal cancer patients

Journal of Gastrointestinal Cancer volume 52, pages 1016–1021 (2021)Cite this article

Abstract

Introduction

The direct association between some microbial species and cancers, such as in colorectal cancer (CRC), has been disclosed.

Objective

The aim of this study was to evaluate the changes in intestinal microbiota in subjects with CRC compared with healthy group.

Methods

Three-hundred fecal specimens were gathered from patients with CRC and 300 from healthy individuals during March 2014 to October 2019 from two hospitals in Tehran. The informed consent form and the questionnaire were completed by the patients. Following the identification of Lactobaccilus acidophilus (L. acidophilus), Lactobacillus palntarom (L. palntarom), and Enterococcus faecalis (E. faecalis), the number of bacteria was determined using quantitative real-time PCR (qPCR).

Results

The patients’ age range was 20–76 years (mean: 55.34 ± 3.66). The qPCR clarified that number of E. faecalis was 2.2-fold higher in patients with CRC compared to healthy population (p = 0.0013). Additionally, the number of L. acidophilus and L. plantarom was 3.4-fold (p < 0.0001) and 4.8-fold (p < 0.0001) higher in healthy population.

Conclusion

The inhibitory effect of intestinal microflora against the CRC development was proposed by observation of the changes in intestinal microbiota and determining their composition in subjects with CRC compared with that of healthy individuals. Microbiota was considered as a goal for the prevention and treatment of CRC. The relationship between microbiota and human health would be known deeper; this knowledge provides insights into the management of intestinal microbiota and therapeutics.

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Author information

Affiliations

  1. Department of Environment, College of Science, University of Al-Qadisiyah, Diwaniyah, Iraq

    Tha’ir Abid D’asheesh

  2. Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq

    Bashdar Mahmud Hussen

  3. Department of Biology, College of Science for Women, University of Babylon, Hillah, Iraq

    Ali H. Al-Marzoqi

  4. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

    Abdolmajid Ghasemian

Authors
  1. Tha’ir Abid D’asheesh
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  2. Bashdar Mahmud Hussen
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  3. Ali H. Al-Marzoqi
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  4. Abdolmajid Ghasemian
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Correspondence to Abdolmajid Ghasemian.

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D’asheesh, T.A., Hussen, B.M., Al-Marzoqi, A.H. et al. Assessment of oncogenic role of intestinal microbiota in colorectal cancer patients. J Gastrointest Canc 52, 1016–1021 (2021). https://doi.org/10.1007/s12029-020-00531-8

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  • DOI: https://doi.org/10.1007/s12029-020-00531-8

Keywords

  • Lactobacillus spp
  • Enterococcus faecalis
  • colorectal cancer