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Anticancer Impacts of Terminalia catappa Extract on SW480 Colorectal Neoplasm Cell Line

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Abstract

Purpose

Colorectal cancer (CRC) is one of the most lethal and prevalent cancers throughout the world. Despite the remarkable advance in the field, drug resistance still remains as an unresolved problem in cancer. Hence, finding effective compounds with minimal side effects to fight cancer is of central priority. Herbal products have been traditionally used to prevent and treat a variety of diseases.

Methods

In the present study, the antitumor effect of Terminalia catappa plant ethanolic extract (TCE) was assessed on SW480 CRC model cell line. In this regard, effects of TCE were evaluated on the proliferation, apoptosis, and migration of SW480 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Annexin V/PI flow cytometry, and scratch tests, respectively. Furthermore, changes in the expression of genes involved in these events including Bax, Bcl-2, Caspase 3, Caspase 8, Caspase 9, MMP-13, miR-21, and miR-34a were measured by quantitative real-time PCR (qRT-PCR).

Results

According to the MTT results, TCE reduced the proliferation of SW480 cells significantly. The flow cytometry test also revealed a notable rate of apoptosis induction after TCE treatment. An inhibitory effect on cell migration was also evident in scratch test. Expression patterns of the assessed genes also changed subsequent to TCE treatment.

Conclusion

The results of this study indicated that T. catappa could be considered as a potential source of anticancer compounds and a candidate for further investigations.

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Funding

This study was funded by the Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Science, Tabriz, Iran.

Author information

Authors and Affiliations

  1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Dariush Shanehbandi & Milad Asadi

  2. Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Science, Daneshgah Street, Tabriz, Iran

    Habib Zarredar, Venus Zafari, Shiva Esmaeili, Ensiyeh Seyedrezazadeh, Zahra Soleimani, Hamed Sabagh Jadid, Shirin Eyvazi, Sara Feyziniya & Majid Khalili

  3. Students Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Habib Zarredar

  4. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Sedigheh Bamdad Moghadam

Authors
  1. Dariush Shanehbandi
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  2. Habib Zarredar
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  3. Milad Asadi
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  4. Venus Zafari
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  5. Shiva Esmaeili
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  6. Ensiyeh Seyedrezazadeh
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  7. Zahra Soleimani
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  8. Hamed Sabagh Jadid
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  9. Shirin Eyvazi
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  11. Sedigheh Bamdad Moghadam
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  12. Majid Khalili
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Corresponding author

Correspondence to Majid Khalili.

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The authors declare that they have no conflict of interests.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Shanehbandi, D., Zarredar, H., Asadi, M. et al. Anticancer Impacts of Terminalia catappa Extract on SW480 Colorectal Neoplasm Cell Line. J Gastrointest Canc 52, 99–105 (2021). https://doi.org/10.1007/s12029-019-00349-z

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  • DOI: https://doi.org/10.1007/s12029-019-00349-z

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