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Overexpression and Clinicopathological Correlation of Long Noncoding RNA TMPO-AS1 in Colorectal Cancer Patients

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Abstract

Purpose

Long noncoding RNA (lncRNA) has been identified as an important modulator of gene expression and other activities of the cells. This kind of genes also has a significant role in the growth and development of human cancers, including colorectal cancer (CRC). Among lncRNAs, thymopoietin (TMPO)-antisense RNA 1 (TMPO-AS1) is of particular significance and might have a role in CRC regulation. The current study aimed to determine the involvement of TMPO-AS1 in CRC patients.

Methods

In this study, 50 pairs of tumor and tumor marginal samples of CRC patients were investigated to assess the expression level of TMPO-AS1 in this cancer. For this purpose, the total RNA was isolated from the tissues using the TRIzol RNA extraction method, and after synthesis of the complementary DNA, the TMPO-AS1 expression was measured by quantitative real-time PCR (qRT-PCR) technique. In addition, clinicopathological characteristics of the CRC patients were analyzed in the study groups.

Results

The findings demonstrated the overexpression of TMPO-AS1 in CRC tissues. Interestingly, the expression level of TMPO-AS1 was significantly correlated with clinicopathological features of the patients such as lymph node and distant metastasis.

Conclusion

The overexpression of TMPO-AS1 gene in CRC suggests that this lncRNA and its underlying signaling pathways can be considered a prognostic tumor marker and may pave the way for the future development of novel therapeutic options for CRC.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

References

  1. Siegel RL, Miller KD, Fedewa SA, Ahnen DJ, Meester RGS, Barzi A, et al. Colorectal cancer statistics, 2017. CA Cancer J Clin. 2017;67:177–93.

    Article  Google Scholar 

  2. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-tieulent J, Jemal A. Global Cancer statistics, 2012. CA a cancer J Clin. 2015;65:87–108.

    Article  Google Scholar 

  3. Shirafkan N, Mansoori B, Mohammadi A, Shomali N, Ghasbi M, Baradaran B. MicroRNAs as novel biomarkers for colorectal cancer: new outlooks. Biomed Pharmacother Elsevier. 2018;97:1319–30.

    Article  CAS  Google Scholar 

  4. Haggar FA, Boushey RP. Colorectal cancer epidemiology: incidence, mortality, survival, and risk factors. Clin Colon Rectal Surg. 2009;22:191–7.

    Article  Google Scholar 

  5. Sadreddini S, Safaralizadeh R, Baradaran B, Aghebati-Maleki L, Hosseinpour-Feizi MA, Shanehbandi D, et al. Chitosan nanoparticles as a dual drug/siRNA delivery system for treatment of colorectal cancer. Immunol Lett. Elsevier B.V.; 2017;181:79–86.

  6. Hallaj-Nezhadi S, Valizadeh H, Dastmalchi S, Baradaran B, Jalali MB, Dobakhti F, et al. Preparation of chitosan-plasmid DNA nanoparticles encoding interleukin-12 and their expression in CT-26 colon carcinoma cells. J Pharm Pharm Sci. 2011;14:181–95.

    Article  CAS  Google Scholar 

  7. Xie F, Xiang X, Huang Q, Ran P, Yuan Y, Li Q, et al. Reciprocal control of lncRNA-BCAT1 and β-catenin pathway reveals lncRNA-BCAT1 long non-coding RNA acts as a tumor suppressor in colorectal cancer. Oncotarget. 2017;8:23628–37.

    Article  Google Scholar 

  8. Kazemzadeh M, Safaralizadeh R, Feizi MAH, Somi MH, Shokoohi B. Misregulation of the dependence receptor DCC and its upstream lincRNA, LOC100287225, in colorectal cancer. Tumori J. 2017;103:40–3.

    Article  CAS  Google Scholar 

  9. Ponting CP, Oliver PL, Reik W. Evolution and functions of long noncoding RNAs. Cell. Elsevier Inc.; 2009;136:629–41.

  10. Peng WX, Koirala P, Mo YY. LncRNA-mediated regulation of cell signaling in cancer. Oncogene. 2017;36:5661–7.

    Article  CAS  Google Scholar 

  11. Jarroux J, Morillon A, Pinskaya M. History, discovery, and classification of lncRNAs. Adv Exp Med Biol. 2017. p. 1–46.

  12. Brosnan CA, Voinnet O. The long and the short of noncoding RNAs. Curr Opin Cell Biol. 2009;21:416–25.

    Article  CAS  Google Scholar 

  13. Huang W, Su X, Yan W, Kong Z, Wang D, Huang Y, et al. Overexpression of AR-regulated lncRNA TMPO-AS1 correlates with tumor progression and poor prognosis in prostate cancer. Prostate. 2018;78:1248–61.

    Article  CAS  Google Scholar 

  14. Peng F, Wang R, Zhang Y, Zhao Z, Zhou W, Chang Z, et al. Differential expression analysis at the individual level reveals a lncRNA prognostic signature for lung adenocarcinoma. Mol Cancer Molecular Cancer. 2017;16:1–12.

    Article  CAS  Google Scholar 

  15. Zhang L, Wang G, Chen S, Ding J, Ju S, Cao H, et al. Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells. World J Surg Oncol World Journal of Surgical Oncology. 2016;14:1–8.

    CAS  Google Scholar 

  16. Brachner A, Foisner R. Lamina-associated polypeptide (LAP)2α and other LEM proteins in cancer biology. 2014. p. 143–63.

  17. Siahmansouri H, Somi MH, Babaloo Z, Baradaran B, Jadidi-Niaragh F, Atyabi F, et al. Effects of HMGA2 siRNA and doxorubicin dual delivery by chitosan nanoparticles on cytotoxicity and gene expression of HT-29 colorectal cancer cell line. J Pharm Pharmacol. 2016:1119–30.

  18. Daryabari SS, Safaralizadeh R, Hosseinpourfeizi M, Moaddab Y, Shokouhi B. Overexpression of SSH1 in gastric adenocarcinoma and its correlation with clinicopathological features. J Gastrointest Oncol. 2018;9:728–33.

    Article  Google Scholar 

  19. Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A, et al. Landscape of transcription in human cells. Nature. 2012;489:101–8.

    Article  CAS  Google Scholar 

  20. Ong MS, Cai W, Tan TZ, Huang RY, Hooi SC, Yap CT, et al. Long non-coding RNA landscape in colorectal cancer. Rna Dis. 2018;5:1–9.

    Google Scholar 

  21. Bhan A, Soleimani M, Mandal SS. Long noncoding RNA and cancer: a new paradigm. Cancer Res. 2017;77:3965–81.

    Article  CAS  Google Scholar 

  22. Chen G, Wang Z, Wang D, Qiu C, Liu M, Chen X, et al. LncRNA disease: a database for long-non-coding RNA-associated diseases. Nucleic Acids Res. 2013;41:983–6.

    Article  Google Scholar 

  23. Schmitt AM, Chang HY. Long noncoding rnas in cancer pathways. Cancer Cell. Elsevier Inc.; 2016;29:452–63.

  24. Qin Z, Zheng X, Fang Y. Long noncoding RNA TMPO-AS1 promotes progression of non-small cell lung cancer through regulating its natural antisense transcript TMPO. Biochem Biophys Res Commun Elsevier Ltd. 2019;516:486–93.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank the Immunology Research Center, Tabriz University of Medical Sciences, for providing facilities to carry out this work.

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Authors and Affiliations

  1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Haniye Mohammadrezakhani, Behzad Baradaran, Dariush Shanehbandi, Milad Asadi & Khalil Hajiasgharzadeh

  2. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Haniye Mohammadrezakhani & Reza Safaralizadeh

  3. Department of Thoracic Surgery, Tabriz University of Medical Sciences, Tabriz, Iran

    Shahriar Hashemzadeh

Authors
  1. Haniye Mohammadrezakhani
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  2. Behzad Baradaran
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  3. Dariush Shanehbandi
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  4. Milad Asadi
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  5. Shahriar Hashemzadeh
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  6. Khalil Hajiasgharzadeh
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  7. Reza Safaralizadeh
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Contributions

HM, BB, DS, MA, and SH performed the experiments. BB provided biological materials and reagents. HM and KH wrote the article. HM, DS, MA, and KH performed data analysis. BB and RS participated in the design of the work. RS supervised the study.

Corresponding author

Correspondence to Reza Safaralizadeh.

Ethics declarations

Written informed consent was obtained from all participants for their tissues to be utilized in this research. The study was approved by the ethical committee of Tabriz University of Medical Sciences, Tabriz, Iran.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Mohammadrezakhani, H., Baradaran, B., Shanehbandi, D. et al. Overexpression and Clinicopathological Correlation of Long Noncoding RNA TMPO-AS1 in Colorectal Cancer Patients. J Gastrointest Canc 51, 952–956 (2020). https://doi.org/10.1007/s12029-019-00333-7

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  • DOI: https://doi.org/10.1007/s12029-019-00333-7

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