Abstract
Purpose
One of the worst types of cancers is gastric cancer and no specific tumor marker is found in relation to it. Reactive oxygen species modulator 1 (ROMO1) and the overlapping with the M-AAA protease 1 homolog (OMA1) proteins are the most important mitochondrial membrane proteins, which are involved in modulating reactive oxygen species (ROS) production and the regulation of mitochondrial structure dynamics. If these proteins do not function properly, oxidative stress increases in the cell, and this can initiate the cancer or worsen the condition.
Methods
In this study, ROMO1 and OMA1 gene expressions in 40 fresh frozen gastric cancer tissue and healthy adjacent tissues were evaluated by real-time PCR, and some of the important parameters related to oxidative stress such as TAC, TOS, MDA, and TTG in the serum of cancer patients compared to healthy people were measured by spectrophotometric and fluorometric techniques.
Results
We observed that ROMO1 and OMA1 gene expressions in gastric cancer tissues increased compared to that in healthy adjacent tissues. In addition, oxidative stress parameters including TOS, OSI, and MDA in the serum of cancer patients have increased significantly and the parameters including TAC and TTG have decreased.
Conclusion
The results in our study represented that ROMO1 and OMA1 gene expressions in gastric cancer tissue have increased compared to that in healthy adjacent tissues, and oxidative stress levels have also increased significantly in relation to these proteins; therefore, these two proteins may be considered as an important cause of gastric cancer, and even introduced as tumor markers.
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Acknowledgments
We would like to thank H. Moridi and H. Rezaie and MR. Seyed Rahmani for their assistance. The results presented in this article were part of M.A. Amini MSc thesis.
Funding
This study was financially supported by Hamadan University of Medical Sciences (No: 9709135418).
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The study protocol was approved by Hamadan University of Medical Sciences Ethics Committee (code: IR.UMSHA.REC.1397.506 approved on 4 Dec 2018).
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The authors declare that they have no conflicts of interest.
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Amini, M.A., Karimi, J., Khodadadi, I. et al. Overexpression of ROMO1 and OMA1 are Potentially Biomarkers and Predict Unfavorable Prognosis in Gastric Cancer. J Gastrointest Canc 51, 939–946 (2020). https://doi.org/10.1007/s12029-019-00330-w
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DOI: https://doi.org/10.1007/s12029-019-00330-w