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Assessment of MMP29 Gene Expression and Silver Nanoparticles Effects on Colon Cancer Cell Line (HT29)



Colon cancer is a major cause of death around the world. The evaluation of novel approaches on corresponding genes would be a vital strategy toward eradication of cancer cells. In this study, the toxicity of silver nanoparticle on the colon cancer cell line (HT29) and expression of matrix metalloproteinase (MMP29) gene was investigated.

Materials and Methods

The silver nanoparticle (AgNPs) was synthesized and assessed by transmission electron microscopy (TEM). The cytotoxicity of synthesized AgNP on the HT29 cell line was evaluated using the MTT assay. Furthermore, the expression of MMP29 gene was investigated by the quantitative real-time PCR (RT-qPCR).


The TEM results revealed that the fabricated AgNPs were mostly spherical in shape and had an average diameter of 22 nm. The results outlined that AgNPs significantly decreased the viability of cells in a dose- and time-dependent manner (p < 0.001). Additionally, we observed a significant difference among various concentrations.


The findings indicated that the green fabricated AgNPs have the potential as a promising approach toward the colon cancer therapy. Furthered studies are essential to evaluate against other cancer cell lines and genes participating in the cancer progress.

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  1. 1.

    Senapati S, Syed A, Moeez S, Kumar A, Ahmad A. Intracellular synthesis of gold nanoparticles using alga Tetraselmis kochinensis. Mater Lett. 2012;79:116–8.

    CAS  Article  Google Scholar 

  2. 2.

    Prasad TN, Kambala VSR, Naidu R. Phyconanotechnology: synthesis of silver nanoparticles using brown marine algae Cystophora moniliformis and their characterisation. J Appl Phycol. 2013;25(1):177–82.

    CAS  Article  Google Scholar 

  3. 3.

    Cai F, Li J, Sun J, Ji Y. Biosynthesis of gold nanoparticles by biosorption using Magnetospirillum gryphiswaldense MSR- 1. Chem Eng J. 2011;75(1):70–5.

    Article  Google Scholar 

  4. 4.

    Song JY, Jang HK, Kim BS. Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts. Process Biochem. 2009;44(10):1133–8.

    CAS  Article  Google Scholar 

  5. 5.

    Vanaja M, Annadurai G. Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity. Appl Nanosci. 2013;3(3):217–23.

    CAS  Article  Google Scholar 

  6. 6.

    Rekha A, Arya V. Biological synthesis of silver nanoparticles from aqueous extract of endophytic fungus Aspergillus terrus and its antibacterial activity. Int J Nanomater Biostruc. 2013;2:35–9.

    Google Scholar 

  7. 7.

    Crowe DL, Tsang KJ, Shemirani B. Jun N-terminal kinase 1 mediates transcriptional induction of matrix metal loproteinase 9 expression. Neoplasia. 2001;3(1):27–32.

    CAS  Article  Google Scholar 

  8. 8.

    Gruss C, Herlyn M. Role of cadherins and matrixins in melanoma. Curr Opinion Oncol. 2001;13(2):117–23.

    CAS  Article  Google Scholar 

  9. 9.

    Egeblad M, Werb Z. New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer. 2002;2(3):161–74.

    CAS  Article  Google Scholar 

  10. 10.

    Visse R, Nagase H. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ Res. 2003;92(8):827–39.

    CAS  Article  Google Scholar 

  11. 11.

    Shandiz SAS, Shafiee Ardestani M, Shahbazzadeh D, Assadi A, et al. Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells. Artif Cells Nanomed Biotechnol. 2017;45(6):1082–91.

    Article  Google Scholar 

  12. 12.

    Abbai R, Mathiyalagan R, Markus J, Kim YJ, Wang C, Singh P, et al. Green synthesis of multifunctional silver and gold nanoparticles from the oriental herbal adaptogen: Siberian ginseng. Int J Nanomedicine. 2016;11:3131–43.

    CAS  Article  Google Scholar 

  13. 13.

    Wan G, Ruan L, Yin Y, Yang T, Ge M, Cheng X. Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii. Int J Nanomedicine. 2016;11:3789–800.

    CAS  Article  Google Scholar 

  14. 14.

    Devi J, Bhimba B. Anticancer activity of silver nanoparticles synthesized by the seaweed Ulva lactuca in vitro. Sci Rep. 2012;1(4):242.

    Google Scholar 

  15. 15.

    Salehi S, Shandiz SAS, Ghanbar F, Darvish MR, et al. Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. Int J Nanomedicine. 2016;11:1835–46.

    CAS  PubMed  PubMed Central  Google Scholar 

  16. 16.

    Khatami M, Kharazi S, Kishani Farahani Z, Azizi H, et al. The anti-cancer effect of octagon and spherical silver nanoparticles on MCF-7 breast cancer cell line. Tehran Univ Med J. 2017;75(1):72–6.

    Google Scholar 

  17. 17.

    Mata R, Nakkala JR, Sadras SR. Biogenic silver nanoparticles from Abutilon indicum: their antioxidant, antibacterial and cytotoxic effects in vitro. Colloid Surface B. 2015;128:276–86.

    CAS  Article  Google Scholar 

  18. 18.

    Williams DB, Carter CB. X-ray spectrometry. Transmission Electron Microscopy. 2009:581–603.

  19. 19.

    Kirman I, Jain S, Cekic V, Belizon A, Balik E, Sylla P, et al. Altered plasma matrix metalloproteinase-9/tissue metallpproteinase metalloproteinase 1 concentration during the early postoperative period in patients with colorectal cancer. Surg Endosc. 2006;20(3):482–6.

    CAS  Article  Google Scholar 

  20. 20.

    Emara M, Cheung PY, Grabowski K, Sawicki G, Wozniak M. Serum levels of matrix metalloproteinase-2 and-9 and conventional tumor markers (CEA and CA 19-9) in patients with colorectal and gastric cancers. Clin Chem Lab Med. 2009;47(8):993–1000.

    CAS  Article  Google Scholar 

  21. 21.

    Ławicki S, Zajkowska M, Głażewska EK, Będkowska GE, et al. Plasma levels and diagnostic utility of VEGF, MMP-9, and TIMP-1 in the diagnosis of patients with breast cancer. Onco Targets Ther. 2016;9:911–9.

    PubMed  PubMed Central  Google Scholar 

  22. 22.

    Yulius S, Asroel HA, Aboet A, Zaluchu FJBMJ. Correlation of Matrix Metalloproteinase-9 (MMP-9) expression and bone destruction in Chronic Suppurative Otitis Media (CSOM) patients with cholesteatoma at Adam Malik General Medan-Indonesia. Bali Med J. 2018;7:195–200.

    Article  Google Scholar 

  23. 23.

    Baugh MD, Perry MJ, Hollander AP, Davies DR, Cross SS, Lobo AJ, et al. Matrix metalloproteinase levels are elevated in inflammatory bowel disease. Gastroenterology. 1999;117(4):814–22.

    CAS  Article  Google Scholar 

  24. 24.

    Zucker S, Hymowitz M, Conner C, Zarrabi HM, et al. Measurement of matrix metalloproteinases and tissue inhibitors of metalloproteinases in blood and tissues: clinical and experimental applications. Ann N Y Acad Sci. 1999;878:212–27.

    CAS  Article  Google Scholar 

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Correspondence to Abdolmajid Ghasemian.

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Ali, A.N.M., Kareem, S.M. & Ghasemian, A. Assessment of MMP29 Gene Expression and Silver Nanoparticles Effects on Colon Cancer Cell Line (HT29). J Gastrointest Canc 51, 560–563 (2020).

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  • AgNPs
  • HT29
  • Matrix metalloproteinase 9
  • Colon Cancer
  • Cytotoxicity