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Evaluation of the Methylation of MIR129-2 Gene in Gastric Cancer

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Abstract

Background

Genetic and epigenetic changes have strong role in the development of gastric cancer. The mutation of the MIR129-2 gene is one of the major causes in many cancers, especially gastric cancer. The aim of this study was to investigate the methylation changes of the MIR129-2 gene in tumor and normal tissue of patients with gastric cancer.

Method

In this study, 50 gastric cancer patients with Iranian Azari ethnic origin without any familial relations were included. Genomic DNAs was extracted from the tumoral and normal tissues. Then the promotor regions of the MIR129-2 gene were analyzed by methylation-specific PCR (MSP) to evaluate the presence or absence of methylated CpG sites.

Results

There was a statistically significant difference in methylation level of MIR129-2 gene between tumoral and normal tissues. It was observed that 84 out of 100 CpG cites were methylated in tumoral tissues in compression to 13 out of 100 CpG cites in normal tissues.

Conclusion

MIR129-2 gene was hypermethylated in tumoral tissues, suggesting that methylation is involved in the development of gastric cancer.

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Funding

This project was supported by a grant from research committee of Tabriz Medical University, Tabriz, Iran.

Author information

Author notes

  1. Nazila Alizadeh and Milad Asadi contributed equally to this work.

Authors and Affiliations

  1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Nazila Alizadeh, Milad Asadi & Dariush Shanehbandi

  2. Tuberculosis and Lung Disease Research Center, Tabriz University of Medical sciences, Tabriz, Iran

    Venus Zafari

  3. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Navid Shomali

  4. Department of Surgery, Tabriz University of Medical sciences, Tabriz, Iran

    Touraj Asvadi

  5. Liver and Gastrointestinal disease Research Center, Tabriz University of Medical sciences, Tabriz, Iran

    Bita Sepehri

Authors
  1. Nazila Alizadeh
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  2. Milad Asadi
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  3. Dariush Shanehbandi
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  4. Venus Zafari
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  5. Navid Shomali
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  6. Touraj Asvadi
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  7. Bita Sepehri
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Corresponding author

Correspondence to Bita Sepehri.

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Ethics Approval and Consent to Participate

Informed consent was obtained from each patient and the ethical approaches were approved by the ethic committee of Tabriz University of Medical Sciences.

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The authors declare that they have no conflict of interests.

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Alizadeh, N., Asadi, M., Shanehbandi, D. et al. Evaluation of the Methylation of MIR129-2 Gene in Gastric Cancer. J Gastrointest Canc 51, 267–270 (2020). https://doi.org/10.1007/s12029-019-00239-4

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  • DOI: https://doi.org/10.1007/s12029-019-00239-4

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