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Increased Expression of MiR-27a and MiR-24-2 in Esophageal Squamous Cell Carcinoma

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Abstract

Purpose

Esophageal squamous cell carcinoma (ESCC) is one of the predominant types of esophageal cancer with poor prognosis which shows high prevalence in eastern countries. Studying microRNAs that were considered for their capabilities such as tissue-specific expression and involvement in different cell features may be informative in the field of diagnostic and prognostic tumor markers. The expression levels of miR-27a and miR-24-2 have been reported to be dysregulated in various cancers and contribute in tumorigenesis and progression; thus, evaluating their expressional behavior and its association with tumor states alteration in ESCC could potentially be helpful.

Methods

The study was conducted on 30 fresh specimens including tumor and normal counterparts’ tissues of ESCC. After the extraction of total RNA, complementary DNA synthesis was performed by the use of linear specific primers. Eventually, real-time polymerase chain reaction was carried out for the measurement of microRNAs expression level.

Results

According to the obtained data, miR 27a and miR-24-2 were significantly upregulated (~2.5 fold, p < 0.05) in tumor specimens compared with their normal adjacent tissue; Moreover, upregulation of miR-27a and 24-2 showed cooperative relationship while analyzed. However, there was no correlation between clinicopathological features and microRNAs upregulation.

Conclusions

The results of this study show that miR-27a and miR-24-2 cooperatively upregulate in ESCC and suggest that these microRNAs can be introduced as a candidate for further study in the field of screening and prognostic biomarkers.

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Acknowledgments

This research was supported by the Shahid Sadoughi University of Medical Sciences. We are also grateful to the Golestan Research Center of Gastroenterology and Hepatology for their help in collecting the samples.

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Authors and Affiliations

  1. Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Mohaddese Maghsudlu & Ehsan Farashahi Yazd

  2. Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Mohaddese Maghsudlu

  3. Sayad Shirazi Hospital, Golestan Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran

    Taghi Amiriani

Authors
  1. Mohaddese Maghsudlu
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  2. Ehsan Farashahi Yazd
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  3. Taghi Amiriani
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Correspondence to Ehsan Farashahi Yazd.

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Ethical Standards

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki declaration of 1964 and later versions. Informed consent or substitute for it was obtained from all patients for being included in the study.

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The authors declare that they have no conflict of interest.

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Maghsudlu, M., Farashahi Yazd, E. & Amiriani, T. Increased Expression of MiR-27a and MiR-24-2 in Esophageal Squamous Cell Carcinoma. J Gastrointest Canc 51, 227–233 (2020). https://doi.org/10.1007/s12029-019-00232-x

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  • DOI: https://doi.org/10.1007/s12029-019-00232-x

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