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Assessment of Relationship Between Expression of Survivin Protein and Histopathology Diagnosis and Malignancy Severity in Colon Specimen

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Abstract

Background

Survivin is a member of the inhibitor of an apoptosis protein family that has been shown to inhibit apoptosis, promote cell proliferation and enhance angiogenesis. In this study, the survivin protein expression in normal, colon polyp, and adenocarcinoma tissues was investigated.

Methods

Immunohistochemical staining for nuclear survivin was carried out on 45 normal colon tissue samples, 38 samples of a colonic polyp, and 37 cases of colon adenocarcinoma operated by colonoscopy or colectomy. The percentages of cells that expressed survivin were classified qualitatively into four categories (0, 1+, 2+, and 3+) based on the intensity of staining and the percentage of cells. An area of samples with colon polyp diagnosis or colon adenocarcinoma that had no microscopic pathology was considered as normal tissues.

Results

Survivin protein expression was negative in all cases of normal colon tissue samples while it was expressed in 31 out of 38 colon polyp specimens (81.5%) and in 35 out of 37 (94.5%) colon adenocarcinoma samples. Amount of expression in the colon adenocarcinoma (p < 0.001) was significantly higher than the amount of expression in the colon polyp. There was not a significant correlation between the survivin protein expression and the low and high grade adenocarcinoma (p = 0.874).

Conclusions

Survivin protein was not expressed in normal colon tissues and its amount was higher in the colonic adenocarcinoma compared to the colon polyp. Due to the variations in the intensity of expression in colon polyp (changing from negative to + 3), this marker cannot be used for differentiating the polyp from the adenocarcinoma.

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Acknowledgments

This work was supported by Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran.

Availability of Data and Materials

The dataset used in this study is available with the authors and can be made available upon request.

Funding

The financial support for the current research was provided by Research Deputy of Kashan University of Medical Sciences, Kashan, Iran.

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Authors and Affiliations

  1. Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran

    Amin Jourabchin, Hamed Haddad Kashani & Tahereh Khamechian

  2. Gametogenese Research Center, Kashan University of Medical Sciences, Kashan, Iran

    Tahereh Mazoochi

Authors
  1. Amin Jourabchin
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  2. Tahereh Mazoochi
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  3. Hamed Haddad Kashani
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  4. Tahereh Khamechian
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Contributions

All the authors participated in the study design. TK, AJ, and TM collected and documented the data and assisted in preliminary data analysis. TK, AJ, and TM wrote the initial draft. HHK participated in draft revision, data analysis, and editing of the final draft.

Corresponding author

Correspondence to Tahereh Khamechian.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Ethics Approval and Consent to Participate

All procedures performed in the study involving human were in accordance with the 1964 Helsinki declaration and ethical standards of the institutional and national research committee of Kashan University of Medical Sciences. The protocol was approved by the research committee of Kashan University of Medical Sciences, Kashan, Iran.

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Jourabchin, A., Mazoochi, T., Haddad Kashani, H. et al. Assessment of Relationship Between Expression of Survivin Protein and Histopathology Diagnosis and Malignancy Severity in Colon Specimen. J Gastrointest Canc 51, 76–82 (2020). https://doi.org/10.1007/s12029-019-00206-z

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  • DOI: https://doi.org/10.1007/s12029-019-00206-z

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