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Transcript Level of MicroRNA Processing Elements in Gastric Cancer

Journal of Gastrointestinal Cancer volume 50, pages 855–859 (2019)Cite this article

Abstract

Background

Aberrant expression of microRNAs (miRNAs) has been implicated in the etiopathogenesis and development of various cancers. Drosha and Dicer are the main components of the miRNA biosynthesis machine. Another enzyme, DGCR8, is the assistant of Drosha in the processing complex. Here, we tried to evaluate the mRNA transcript level of Drosha, Dicer, and DGCR8 genes in involved tissues from patients with gastric cancer.

Methods

Fifty tumoral and their marginal tissues, as the control group, were obtained from patients with gastric cancer. After RNA extraction from tissues and cDNA synthesis, quantification of mRNA expression of Drosha, Dicer, and DGCR8 was conducted using SYBR Green master mix and real-time PCR.

Results

It was observed that mRNA expression levels of Drosha, Dicer, and DGCR8 were significantly upregulated in tumoral tissues compared with marginal tissues. Upregulation of these genes was not correlated with clinical manifestations of the patients.

Conclusions

Upregulation of Drosha, Dicer, and DGCR8 plays a role in the development of cancer, probably through dysregulated the expression level of miRNAs.

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Acknowledgements

We are deeply thankful of our patients for their contribution.

Funding

This study was supported by a grant from Tabriz University of Medical Sciences (Grant No. 94-06-21-4112).

Author information

Affiliations

  1. Research Centers for Liver and Gastrointestinal Disease, Tabriz University of Medical Sciences, Tabriz, Iran

    Milad Asadi & Mohammad Hosein Somi

  2. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Milad Asadi, Dariush Shanehbandi & Vahid Khaze

  3. Tuberculosis and Pulmonary Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Venus Zafari

  4. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Shahriar Hashemzadeh

  5. Department of General and Vascular Surgery, Imam Reza Educational Hospital, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Shahriar Hashemzadeh

Authors
  1. Milad Asadi
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  2. Dariush Shanehbandi
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  3. Venus Zafari
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  4. Vahid Khaze
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  5. Mohammad Hosein Somi
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  6. Shahriar Hashemzadeh
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Corresponding author

Correspondence to Shahriar Hashemzadeh.

Ethics declarations

The Human Research Ethics Committees from the Tabriz University of Medical Sciences approved the protocol of this study and written informed consent was taken by all patients.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Cite this article

Asadi, M., Shanehbandi, D., Zafari, V. et al. Transcript Level of MicroRNA Processing Elements in Gastric Cancer. J Gastrointest Canc 50, 855–859 (2019). https://doi.org/10.1007/s12029-018-0154-8

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  • DOI: https://doi.org/10.1007/s12029-018-0154-8

Keywords

  • Drosha
  • Dicer
  • DGCR8
  • Gastric cancer
  • MicroRNA