Journal of Gastrointestinal Cancer

, Volume 50, Issue 1, pp 42–47 | Cite as

Regulatory Fluctuation of WNT16 Gene Expression Is Associated with Human Gastric Adenocarcinoma

  • Seyedeh Elham Norollahi
  • Majid Alipour
  • Ali Rashidy-Pour
  • Ali Akbar SamadaniEmail author
  • Laleh Vahedi Larijani
Original Research



Gastric cancer is one of the most serious and lethal kinds of cancer in the world. It is a multi-step, multi-factor, and elaborated process that is associated to gene abnormal expression. This study intended to investigate the WNT16 gene’s expression in human gastric tumor and the margin tissues of the stomach (normal tissues).


Correspondingly, 40 samples (20 tumoral tissues and 20 non tumoral or margins tissues) were investigated in Imam Khomeini Hospital in Sari City, Mazandaran Province, Iran. In this way, real-time PCR, Taqman assay was employed to evaluate the upregulation and downregulation of this gene in both tissues in triplicate form. The GAPDH gene was selected as housekeeping gene.


Conspicuously, the results have shown a remarkable modification in tumoral tissues, and the gene expression increased significantly in tumoral tissue.


Conclusively, the upregulation of WNTt16 gene expression in tumoral tissues was impressive and the P value was 0.005 and the SE range was 0.064–142.154.


WNT16 Gene expression Gastric cancer Fluctuation 



This work was the results of Seyedeh Elham Norollahi’s post graduation thesis in the Azad University of Babol Branch and also was confirmed ethically at the related department (1394.93). We appreciate Dr. Maryam Ghasemi in the Pathology Department of Mazandaran Medical Sciences University, Dr. Abbas Ghaheri in Surgery Department of Imam Khomeini Hospital in Sari, and also, all staffs of the Genetic and Biotechnology Research Center of Sari University.

Authors’ contributions

SEN collected data, specimens and done molecular and technical experiments. LVL as a pathologist confirmed, controlled, and managed all the specimens and also all patients’ histopathological qualifications. MA, A R-P, and AAS accomplished the data quality control, investigated and optimized the informatics database, accomplished the statistical analyses and data processing, wrote the paper and evaluated it, did some other scientific activities including study design, project and protocol development, data analysis, and edited the article. All authors revised the manuscript carefully and gave their assistance in order to progress the article. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Seyedeh Elham Norollahi
    • 1
  • Majid Alipour
    • 2
  • Ali Rashidy-Pour
    • 3
    • 4
  • Ali Akbar Samadani
    • 1
    • 3
    Email author
  • Laleh Vahedi Larijani
    • 5
  1. 1.Faculty of Medicine, Cancer Research CenterSemnan University of Medical SciencesSemnanIran
  2. 2.Department of BiologyIslamic Azad University of BabolBabolIran
  3. 3.Faculty of Medicine, Research Center of PhysiologySemnan University of Medical SciencesSemnanIran
  4. 4.Faculty of Medicine, Department of PhysiologySemnan University of Medical SciencesSemnanIran
  5. 5.Faculty of Medicine, Department of PathologyMazandaran University of Medical SciencesSariIran

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