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HES1 as an Independent Prognostic Marker in Esophageal Squamous Cell Carcinoma

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Abstract

Background

Notch signaling is one of the main involved pathways in cell differentiation and organogenesis, and its deregulation may lead to tumorigenesis. In this pathway, targeted to the CSL (CBF1, Suppressor of Hairless or Lag-1) complex, notch intracellular domain (NICD) releases corepressors and recruits MAML1 as coactivator triggering the activation of notch signaling transcription complex. Hairy enhance of split-1 (HES1) is one of the notch signaling target genes which is a basic helix-loop-helix (bHLH) transcription factor acting as a proliferation stimulator through the suppression of cell cycle inhibitors such as p27 and p21.

Aims

In this study, we aimed to analyze the role of HES1 in the progression of esophageal squamous cell carcinoma (ESCC).

Methods

Messenger RNA (mRNA) expression of HES1 in fresh tumoral tissues and their margin normal samples were assessed in 50 ESCC patients by real-time polymerase chain reaction (RT-PCR).

Results

Thirteen out of 50 cases (26 %) had HES1 underexpression, while HES1 overexpression was observed only in 4 (8 %) samples. HES1 underexpression was significantly correlated with tumor depth of invasion (P = 0.035).

Conclusion

Although we have not observed any significant correlation between the HES1 expression and notch activation in ESCC, this study is the first report that elucidated the HES1 underexpression in ESCC and revealed its correlation with the invasiveness of ESCC.

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Acknowledgments

The authors gratefully acknowledge the colleagues at the Division of Human Genetics and Department of Surgery and Pathology of Emam Reza and Omid Hospitals (MUMS) for their assistances in gathering and preparing the ESCC samples.

Conflict of Interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, Iran

    Shaghayegh Taleb & Nasim Hayati Roudbari

  2. Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Shaghayegh Taleb & Meysam Moghbeli

  3. Medical Genetics Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Reza Abbaszadegan

  4. Department of Biology, Damghan Branch, Islamic Azad University, Cheshmeh-Ali Boulevard, Sa’dei Square, Damghan, P.O. Box: 3671639998, Iran

    Mohammad Mahdi Forghanifard

Authors
  1. Shaghayegh Taleb
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  2. Mohammad Reza Abbaszadegan
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  3. Meysam Moghbeli
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  4. Nasim Hayati Roudbari
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  5. Mohammad Mahdi Forghanifard
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Correspondence to Mohammad Mahdi Forghanifard.

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Taleb, S., Abbaszadegan, M.R., Moghbeli, M. et al. HES1 as an Independent Prognostic Marker in Esophageal Squamous Cell Carcinoma. J Gastrointest Canc 45, 466–471 (2014). https://doi.org/10.1007/s12029-014-9648-1

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  • DOI: https://doi.org/10.1007/s12029-014-9648-1

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