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High Serum DNA and RNA Oxidative Damage in Non-surviving Patients with Spontaneous Intracerebral Hemorrhage

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Abstract

Purpose

One study found higher leukocytes 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels in patients with spontaneous intracerebral hemorrhage (ICH) than in healthy subjects due to the oxidation of guanosine from deoxyribonucleic acid (DNA). The objective of this study was to determine whether there is an association between oxidative damage of serum DNA and ribonucleic acid (RNA) and mortality in patients with ICH.

Methods

In this observational and prospective study, patients with severe supratentorial ICH (defined as Glasgow Coma Scale < 9) were included from six Intensive Care Units of Spanish hospitals. At the time of severe ICH diagnosis, concentrations in serum of malondialdehyde (as lipid peroxidation biomarker) and of the three oxidized guanine species (OGS) (8-hydroxyguanosine from RNA, 8-hydroxyguanine from DNA or RNA, and 8-OHdG from DNA) were determined. Thirty-day mortality was considered the end-point study.

Results

Serum levels of OGS (p < 0.001) and malondialdehyde (p = 0.002) were higher in non-surviving (n = 46) than in surviving patients (n = 54). There was an association of serum OGS levels with serum malondialdehyde levels (rho = 0.36; p = 0.001) and 30-day mortality (OR = 1.568; 95% CI 1.183–2.078; p = 0.002).

Conclusions

The novel and most important finding of our study was that serum OGS levels in ICH patients are associated with mortality.

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Abbreviations

APACHE-II:

Acute physiology and chronic health evaluation

aPTT:

Activated partial thromboplastin time

CT:

Computed tomography

FIO2 :

Pressure of arterial oxygen/fraction inspired oxygen

GCS:

Glasgow Coma Scale

ICH:

Spontaneous intracerebral hemorrhage

INR:

International normalized ratio

OGS:

Oxidized guanine species

PaO2 :

Pressure of arterial oxygen

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Acknowledgements

This study was supported by a Grant (OA18/011) from Fundación DISA a la Investigación Médica 2017 (Santa Cruz de Tenerife. Spain). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Author information

Authors and Affiliations

  1. Intensive Care Unit, Hospital Universitario de Canarias, Ofra, s/n, La Laguna, 38320, Santa Cruz de Tenerife, Spain

    Leonardo Lorente

  2. Intensive Care Unit, Hospital Universitario Nuestra Señora de Candelaria, Crta del Rosario s/n, 38010, Santa Cruz de Tenerife, Spain

    María M. Martín

  3. Laboratory Department, Hospital Universitario de Canarias, Ofra, s/n, La Laguna, 38320, Santa Cruz de Tenerife, Spain

    Agustín F. González-Rivero & Antonia Pérez-Cejas

  4. Department of Physiology, Faculty of Medicine, University of the La Laguna, Ofra, s/n, La Laguna, 38320, Santa Cruz de Tenerife, Spain

    Pedro Abreu-González

  5. Department of Radiology, Hospital Universitario de Canarias, Ofra, s/n, La Laguna, 38320, Santa Cruz de Tenerife, Spain

    Rafael Sabatel

  6. Intensive Care Unit, Hospital General La Palma, Buenavista de Arriba s/n, Breña Alta, 38713, La Palma, Spain

    Luis Ramos

  7. Intensive Care Unit, Hospital Clínico Universitario de Valencia, Avda, Blasco Ibáñez no. 17-19, 46004, Valencia, Spain

    Mónica Argueso

  8. Intensive Care Unit, Hospital Insular, Plaza Dr, Pasteur s/n, 35016, Las Palmas de Gran Canaria, Spain

    Juan J. Cáceres

  9. Intensive Care Unit, Hospital Universitario Dr. Negrín, Barranco de la Ballena s/n, 35010, Las Palmas de Gran Canaria, Spain

    Jordi Solé-Violán

  10. Research Unit, Hospital Universitario de Canarias, Ofra, s/n, La Laguna, 38320, Santa Cruz de Tenerife, Spain

    Alejandro Jiménez

  11. Department of Neurosurgery, Hospital Universitario de Canarias, Ofra, s/n, La Laguna, 38320, Santa Cruz de Tenerife, Spain

    Victor García-Marín

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  1. Leonardo Lorente
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Contributions

LLo conceived, designed and coordinated the study, participated in acquisition and interpretation of data, and drafted the manuscript. MMM, RS, LR, MA, JJC, JSV, and VGM participated in acquisition of data. AGC and APC carried out the analyses of DNA and RNA oxidative damage. PAG carried out the analyses of malondialdehyde concentrations. AJ participated in the interpretation of data. All authors revised the manuscript critically for important intellectual content and made the final approval of the version to be published.

Corresponding author

Correspondence to Leonardo Lorente.

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Lorente, L., Martín, M.M., González-Rivero, A.F. et al. High Serum DNA and RNA Oxidative Damage in Non-surviving Patients with Spontaneous Intracerebral Hemorrhage. Neurocrit Care 33, 90–96 (2020). https://doi.org/10.1007/s12028-019-00864-8

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