Abstract
Increasing evidences show that circRNAs are associated with some autoimmunity diseases either as a biomarker or therapeutic target. Exosomes containing nucleic acids and proteins are found in sera of series diseases and could serve as either diagnostic or therapeutic target. ANA serves as first common diagnostic test for autoimmunity disease, different ANA staining reflecting different types of autoimmunity disease. Till now, whether different ANA sera exosomes express different circRNAs and relevant ceRNA networks are still shortage of investigation. This study analyzed circRNAs, miRNAs, and their interaction networks in different ANA sera exosomes by high-throughput sequencing. It found no significant difference of total circRNAs and miRNAs amount across different ANA sera exosomes. However, significant differences were found of circRNAs, miRNA constituents, function analysis by KEGG and GO, and their ceRNA networks including miRNA-circRNA and miRNA-mRNA among different ANA sera exosomes, suggesting sera exosome circRNAs as either biomarker or mechanism of autoimmunity diseases.
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References
Zurawska A, Mycko MP, Selmaj KW. Circular RNAs as a novel layer of regulatory mechanism in multiple sclerosis. J Neuroimmunol [Internet]. 2019/06/05. 2019;334:576971. Available from: https://www.ncbi.nlm.nih.gov/pubmed/31163273
Sigdel KR, Cheng A, Wang Y, Duan L, Zhang Y. The emerging functions of long noncoding RNA in immune cells: autoimmune diseases. J Immunol Res [Internet]. 2015/06/20. 2015;2015:848790. Available from: https://www.ncbi.nlm.nih.gov/pubmed/26090502
Xia X, Tang X, Wang S. Roles of CircRNAs in autoimmune diseases. Front Immunol [Internet]. 2019/04/20. 2019;10:639. Available from: https://www.ncbi.nlm.nih.gov/pubmed/31001261
Blin J, Fitzgerald KA. Perspective: The RNA exosome, cytokine gene regulation and links to autoimmunity. Cytokine [Internet]. 2015/04/04. 2015;74:175–80. Available from: https://www.ncbi.nlm.nih.gov/pubmed/25835609
Duan W, Zhang W, Jia J, Lu Q, Eric Gershwin M. Exosomal microRNA in autoimmunity. Cell Mol Immunol [Internet]. 2019/10/31. 2019;16:932–4. Available from: https://www.ncbi.nlm.nih.gov/pubmed/31664221
Satoh M, Vazquez-Del Mercado M, Chan EK. Clinical interpretation of antinuclear antibody tests in systemic rheumatic diseases. Mod Rheumatol [Internet]. 2009/03/12. 2009;19:219–28. Available from: https://www.ncbi.nlm.nih.gov/pubmed/19277826
Ho KT, Reveille JD. The clinical relevance of autoantibodies in scleroderma. Arthritis Res Ther [Internet]. 2003/04/30. 2003;5:80–93. Available from: https://www.ncbi.nlm.nih.gov/pubmed/12718748
Peene I, Meheus L, Veys EM, De Keyser F. Detection and identification of antinuclear antibodies (ANA) in a large and consecutive cohort of serum samples referred for ANA testing. Ann Rheum Dis [Internet]. 2001/11/16. 2001;60:1131–6. Available from: https://www.ncbi.nlm.nih.gov/pubmed/11709455
Hu J, Meng W, Zhang D, Qiu C, Hua L, Xie Q, et al. Th17-relevant cytokines vary with sera of different ANA staining patterns. Int Immunopharmacol [Internet]. 2013/03/20. 2013;15:679–84. Available from: https://www.ncbi.nlm.nih.gov/pubmed/23507192
Conticini E, Sota J, Falsetti P, Bellisai F, Bacarelli MR, Al-Khayyat SG, et al. Anti-dense fine speckled 70 antibodies in primary Sjogren’s syndrome. Clin Exp Rheumatol [Internet]. 2020/06/24. 2020;38 Suppl 1:326. Available from: https://www.ncbi.nlm.nih.gov/pubmed/32573418
Haenisch S, Von Rüden EL, Wahmkow H, Rettenbeck ML, Michler C, Russmann V, et al. miRNA-187–3p-mediated regulation of the KCNK10/TREK-2 potassium channel in a rat epilepsy model. ACS Chem Neurosci [Internet]. ACS Chem Neurosci; 2016 [cited 2022 Mar 9];7:1585–94. Available from: https://pubmed.ncbi.nlm.nih.gov/27609046/
Furue M, Mitoma C, Mitoma H, Tsuji G, Chiba T, Nakahara T, et al. Pathogenesis of systemic sclerosis-current concept and emerging treatments. Immunol Res [Internet]. 2017/05/11. 2017;65:790–7. Available from: https://www.ncbi.nlm.nih.gov/pubmed/28488090
Timlin H, Wu M, Crespo-Bosque M, Geetha D, Ingolia A, Haque U, et al. Clinical characteristics of hydralazine-induced lupus. Cureus [Internet]. 2019/09/10. 2019;11:e4996. Available from: https://www.ncbi.nlm.nih.gov/pubmed/31497427
Brooks SP, Coccia M, Tang HR, Kanuga N, Machesky LM, Bailly M, et al. The Nance-Horan syndrome protein encodes a functional WAVE homology domain (WHD) and is important for co-ordinating actin remodelling and maintaining cell morphology. Hum Mol Genet [Internet]. Hum Mol Genet; 2010 [cited 2022 Mar 12];19:2421–32. Available from: https://pubmed.ncbi.nlm.nih.gov/20332100/
Borja N, Bivona S, Peart LS, Johnson B, Gonzalez J, Barbouth D, et al. Genome sequencing reveals novel noncoding variants in PLA2G6 and LMNB1 causing progressive neurologic disease. Mol Genet genomic Med [Internet]. Mol Genet Genomic Med; 2022 [cited 2022 Mar 12]; Available from: https://pubmed.ncbi.nlm.nih.gov/35247231/
Amadio R, Piperno GM, Benvenuti F. Self-DNA sensing by cGAS-STING and TLR9 in autoimmunity: is the cytoskeleton in control? Front Immunol [Internet]. Frontiers Media S.A.; 2021 [cited 2022 Mar 12];12. Available from: https://pubmed.ncbi.nlm.nih.gov/34084165/
Oh-hora M. Calcium signaling in the development and function of T-lineage cells. Immunol Rev [Internet]. 2009/09/17. 2009;231:210–24. Available from: https://www.ncbi.nlm.nih.gov/pubmed/19754899
Zhang F, Wu L, Qian J, Qu B, Xia S, La T, et al. Identification of the long noncoding RNA NEAT1 as a novel inflammatory regulator acting through MAPK pathway in human lupus. J Autoimmun [Internet]. 2016/08/03. 2016;75:96–104. Available from: https://www.ncbi.nlm.nih.gov/pubmed/27481557
Murakami Y, Tanahashi T, Okada R, Toyoda H, Kumada T, Enomoto M, et al. Comparison of hepatocellular carcinoma miRNA expression profiling as evaluated by next generation sequencing and microarray. PLoS One [Internet]. 2014/09/13. 2014;9:e106314. Available from: https://www.ncbi.nlm.nih.gov/pubmed/25215888
Xiao S, Yang M, Yang H, Chang R, Fang F, Yang L. miR-330–5p targets SPRY2 to promote hepatocellular carcinoma progression via MAPK/ERK signaling. Oncogenesis [Internet]. 2018/11/23. 2018;7:90. Available from: https://www.ncbi.nlm.nih.gov/pubmed/30464168
Trehoux S, Lahdaoui F, Delpu Y, Renaud F, Leteurtre E, Torrisani J, et al. Micro-RNAs miR-29a and miR-330–5p function as tumor suppressors by targeting the MUC1 mucin in pancreatic cancer cells. Biochim Biophys Acta [Internet]. 2015/06/04. 2015;1853:2392–403. Available from: https://www.ncbi.nlm.nih.gov/pubmed/26036346
Pathak E, Bhavya, Mishra D, Atri N, Mishra R. Deciphering the role of microRNAs in BRD4-NUT fusion gene induced NUT midline carcinoma. Bioinformation [Internet]. 2017/07/22. 2017;13:209–13. Available from: https://www.ncbi.nlm.nih.gov/pubmed/28729764
Fabbri E, Montagner G, Bianchi N, Finotti A, Borgatti M, Lampronti I, et al. MicroRNA miR-93–5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells. Oncol Rep [Internet]. 2016/03/18. 2016;35:2866–72. Available from: https://www.ncbi.nlm.nih.gov/pubmed/26986724
Maffioletti E, Cattaneo A, Rosso G, Maina G, Maj C, Gennarelli M, et al. Peripheral whole blood microRNA alterations in major depression and bipolar disorder. J Affect Disord [Internet]. J Affect Disord; 2016 [cited 2022 Mar 12];200:250–8. Available from: https://pubmed.ncbi.nlm.nih.gov/27152760/
Pan C, Sun G, Sha M, Wang P, Gu Y, Ni Q. Investigation of miR-93–5p and its effect on the radiosensitivity of breast cancer. Cell Cycle [Internet]. Cell Cycle; 2021 [cited 2022 Mar 12];20:1173–80. Available from: https://pubmed.ncbi.nlm.nih.gov/34024254/
Su LC, Xu WD, Liu XY, Fu L, Huang AF. Altered expression of circular RNA in primary Sjogren’s syndrome. Clin Rheumatol [Internet]. 2019/08/20. 2019;38:3425–33. Available from: https://www.ncbi.nlm.nih.gov/pubmed/31420809
Li H, Li K, Lai W, Li X, Wang H, Yang J, et al. Comprehensive circular RNA profiles in plasma reveals that circular RNAs can be used as novel biomarkers for systemic lupus erythematosus. Clin Chim Acta [Internet]. 2018/01/24. 2018;480:17–25. Available from: https://www.ncbi.nlm.nih.gov/pubmed/29360436
Wang J, Yan S, Yang J, Lu H, Xu D, Wang Z. Non-coding RNAs in rheumatoid arthritis: from bench to bedside. Front Immunol [Internet]. 2020/02/13. 2019;10:3129. Available from: https://www.ncbi.nlm.nih.gov/pubmed/32047497
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Jinhui Hu: Conceptualization; methodology; writing, review and editing; project administration. Qiuhua Xie: Data curation; writing, original draft preparation; formal analysis. JingYi Wang: Sample collection, resources. Fengxia Xu: Investigation, visualization. Peng Liu: Investigation, validation. Zhicheng Wang: Conceptualization, supervision, project administration.
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Jinhui Hu and Qiuhua Xie contribute equally to this work.
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Hu, J., Xie, Q., Wang, J. et al. Exosome circRNAs and ceRNA network profiles in different ANA sera. Immunol Res 70, 518–529 (2022). https://doi.org/10.1007/s12026-022-09282-z
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DOI: https://doi.org/10.1007/s12026-022-09282-z