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Downregulation of CD163 in monocytes and its soluble form in the plasma is associated with a pro-inflammatory profile in pregnant women with preeclampsia

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Abstract

Preeclampsia (PE) is a pregnancy-specific syndrome characterized by a systemic inflammatory response that polarizes peripheral blood monocytes to the M1 phenotype. The classically activated M1 monocytes comprise immune effector cells with an acute inflammatory phenotype. CD163 is a scavenger receptor expressed by monocytes/macrophages that may be shed from their cell membrane after proteolytic cleavage, producing the soluble CD163 molecule (sCD163). This study evaluated CD163 expression by monocytes and sCD163 as well as pro- and anti-inflammatory cytokine concentration in the plasma of pregnant women with PE. Fifty-six women with PE and 28 normotensive pregnant women were included. Plasma levels of sCD163, interleukin-1 beta (IL-1β), IL-6, IL-10, transforming growth factor beta (TGF-β1), and tumor necrosis factor-alpha (TNF-α) were determined by ELISA, and CD163 expression by monocytes was assessed by flow cytometry. The expression of CD163 by monocytes was significantly lower in severe and mild PE than in normotensive pregnant. Plasma concentrations of IL-1β, TGF-β1, and TNF-α were higher in severe PE than in mild PE and normotensive pregnant women. Both groups of preeclamptic women showed decreased plasma levels of sCD163 and IL-10. Negative correlations between sCD163 and IL-1β (r = − 0.45; P = 0.014) and between sCD163 and TNF-α concentrations (r = − 0.54; P = 0.001) were observed in the severe PE group. The association between the pro-inflammatory cytokine profile and lower concentrations of sCD163 and IL-10 in plasma from women with severe PE suggests an impairment in the modulation of the systemic inflammatory response in this group of pregnant women with preeclampsia.

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Funding

This study was supported by the Fundação de Amparo a Pesquisa do Estado de Sao Paulo (São Paulo State Research Foundation) no. 2010/20207-0 and no. 2013/12260-7.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, São Paulo State University, UNESP, Rubião Junior s/n, Botucatu, São Paulo, 18618-689, Brazil

    Priscila R. Nunes, Mariana Romão-Veiga & Maria Terezinha Peraçoli

  2. Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil

    José C. Peraçoli, Roberto A. Araujo Costa, Leandro G. de Oliveira & Vera Therezinha M. Borges

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  1. Priscila R. Nunes
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  2. Mariana Romão-Veiga
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  3. José C. Peraçoli
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  4. Roberto A. Araujo Costa
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  5. Leandro G. de Oliveira
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  6. Vera Therezinha M. Borges
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  7. Maria Terezinha Peraçoli
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Contributions

M.T.P. and P.R.N. conceived and designed the experiments. P.R.N. and M.R.V. performed the experiments. P.R.N., M.T.P., and M.R.V. analyzed the data and wrote the manuscript. J.C.P., R.A.A.C., L.G.O., and V.T.M.B. recruited the subjects for clinical evaluation and blood collection. All the authors have seen and approved the final version of the manuscript.

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Correspondence to Priscila R. Nunes.

Ethics declarations

This project was approved by the Ethics Committee of the Botucatu Medical School (Protocol number 417.350), and written informed consent was obtained from all women involved in the study.

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The authors declare that they have no conflict of interest.

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Nunes, P.R., Romão-Veiga, M., Peraçoli, J.C. et al. Downregulation of CD163 in monocytes and its soluble form in the plasma is associated with a pro-inflammatory profile in pregnant women with preeclampsia. Immunol Res 67, 194–201 (2019). https://doi.org/10.1007/s12026-019-09078-8

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  • DOI: https://doi.org/10.1007/s12026-019-09078-8

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