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Catalase expression of Propionibacterium acnes may contribute to intracellular persistence of the bacterium in sinus macrophages of lymph nodes affected by sarcoidosis

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Abstract

Bacterial catalase is important for intracellular survival of the bacteria. This protein of Propionibacterium acnes, one of possible causes of sarcoidosis, induces hypersensitive Th1 immune responses in sarcoidosis patients. We examined catalase expression in cultured P. acnes isolated from 19 sarcoid and 18 control lymph nodes and immunohistochemical localization of the protein in lymph nodes from 43 sarcoidosis and 102 control patients using a novel P. acnes-specific antibody (PAC) that reacts with the catalase protein, together with the previously reported P. acnes-specific PAB and TIG antibodies. High catalase expression of P. acnes cells was found during stationary phase in more isolates from sarcoid than from non-sarcoid lymph nodes and was associated with bacterial survival under H2O2-induced oxidative stress. In many sarcoid and some control lymph nodes, catalase expression was detected at the outer margins of PAB-reactive Hamazaki-Wesenberg (HW) bodies in sinus macrophages, the same location as catalase expression on the surface of cultured P. acnes and the same distribution as bacterial cell membrane-bound lipoteichoic acid in HW bodies. Some or no catalase expression was detected in sarcoid granulomas with PAB reactivity or in clustered paracortical macrophages packed with many PAB-reactive small-round bodies. HW bodies expressing catalase may be persistent P. acnes in sinus macrophages whereas PAB-reactive small-round bodies with undetectable catalase may be activated P. acnes proliferating in paracortical macrophages. Intracellular proliferation of P. acnes in paracortical macrophages may lead to granuloma formation by this commensal bacterium in sarcoidosis patients with Th1 hypersensitivity to certain P. acnes antigens, including catalase.

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Acknowledgments

We gratefully acknowledge Dr. Y. Hara and Dr. T. Suzuki for their advice regarding the study design. We thank Ms. Y. Suzuki, Ms. M. Yoshizaki, and Ms. M. Sakaguchi for their technical assistance.

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  1. Kurara Yamamoto and Keisuke Uchida contributed equally to this work.

Authors and Affiliations

  1. Department of Human Pathology, Graduate School and Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 1138510, Japan

    Kurara Yamamoto, Asuka Furukawa, Yuki Ishige, Mariko Negi, Daisuke Kobayashi, Takashi Ito, Tomoya Kakegawa & Yoshinobu Eishi

  2. Division of Surgical Pathology, Tokyo Medical and Dental University Hospital, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 1138510, Japan

    Keisuke Uchida, Tomoki Tamura & Takumi Akashi

  3. Clinical Research Center and Pathology Division, National Hospital Organization Tokyo National Hospital, 3-1-1 Takeoka, Kiyose-si, Tokyo, 2048585, Japan

    Akira Hebisawa

  4. Division of Clinical Respiratory Medicine, Japanese Red Cross Medical Center, 4-1-22 Hiro-o, Shibuya-ku, Tokyo, 1508935, Japan

    Nobuyasu Awano

  5. Division of Pathology, Japanese Red Cross Medical Center, 4-1-22 Hiro-o, Shibuya-ku, Tokyo, 1508935, Japan

    Tamiko Takemura

  6. Division of Pathology, Tokyo Kita Medical Center, 4-17-56 Akabanedai, Kita-ku, Tokyo, 115-0053, Japan

    Tomonari Amano

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  1. Kurara Yamamoto
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Yamamoto, K., Uchida, K., Furukawa, A. et al. Catalase expression of Propionibacterium acnes may contribute to intracellular persistence of the bacterium in sinus macrophages of lymph nodes affected by sarcoidosis. Immunol Res 67, 182–193 (2019). https://doi.org/10.1007/s12026-019-09077-9

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