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Immunologic Research

, Volume 67, Issue 1, pp 84–92 | Cite as

Diminazene aceturate (Berenil) downregulates Trypanosoma congolense-induced proinflammatory cytokine production by altering phosphorylation of MAPK and STAT proteins

  • Shiby M. Kuriakose
  • Chukwunonso Onyilagha
  • Rani Singh
  • Ping Jia
  • Jude E. UzonnaEmail author
Original Article
  • 97 Downloads

Abstract

Diminazene aceturate (Berenil) is the most commonly used trypanolytic agent in livestock. We previously showed that Berenil downregulates Trypanosoma congolense (T. congolense)-induced cytokine production in macrophages both in vitro and in vivo. Here, we investigated the molecular mechanisms through which the drug alters T. congolense-induced cytokine production in macrophages. We show that pretreatment of macrophages with Berenil significantly downregulated T. congolense-induced phosphorylation of mitogen-activated protein kinase (p38), signal transducer and activator of transcription (STAT) proteins including STAT1 and STAT3, and NFκB activity both in vitro and in vivo. Collectively, our results reveal a mechanistic insight through which Berenil downregulates T. congolense-induced cytokine production in macrophages by inhibiting key signaling molecules and pathways associated with proinflammatory cytokine production.

Keywords

Diminazene aceturate (Berenil) Trypanosome Macrophages MAPK STAT1 Proinflammatory cytokines 

Notes

Compliance with ethical standards

The University of Manitoba Animal Care Committee approved all studies in accordance with the regulation of the Canadian Council on Animal Care.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Shiby M. Kuriakose
    • 1
  • Chukwunonso Onyilagha
    • 1
  • Rani Singh
    • 1
  • Ping Jia
    • 1
  • Jude E. Uzonna
    • 1
    Email author
  1. 1.Parasite Vaccines Development Laboratory, Department of Immunology, Max Rady College of Medicine, Rady Faculty of Health SciencesUniversity of ManitobaWinnipegCanada

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