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Phospholipid supplementation can attenuate vaccine-induced depressive-like behavior in mice

Immunologic Research volume 65, pages 99–105 (2017)Cite this article

Abstract

Human papillomavirus vaccine (HPVv) is used worldwide for prevention of infection. However several reports link this vaccine, with immune-mediated reactions, especially with neurological manifestations. Our previous results showed that HPVv-Gardasil and aluminum-immunized mice developed behavioral impairments. Studies have shown a positive effect of phospholipid supplementation on depression and cognitive functions in mice. Therefore, our goal was to evaluate the effect of a dietary supplement on vaccine-induced depression. Sixty C57BL/6 female mice were immunized with HPVv-Gardasil, aluminum or the vehicle (n = 20 each group), and half of each group were fed 5 times per week with 0.2 ml of a dietary supplement enriched with phosphatidylcholine. The mice were evaluated for depression at 3 months of age, by the forced swimming test. Both the Gardasil and the aluminum-treated mice developed depressive-like behavior when compared to the control group. The HPVv-Gardasil-immunized mice supplemented with phosphatidylcholine significantly reduced their depressive symptoms. This study confirms our previous studies demonstrating depressive-like behavior in mice vaccinated with HPVv-Gardasil. In addition, it demonstrates the ability of phosphatidylcholine-enriched diet to attenuate depressive-like behavior in the HPVv-Gardasil-vaccinated mice. We suggest that phosphatidylcholine supplementation may serve as a treatment for patients suffering vaccine-related neurological manifestations.

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Fig. 1

Abbreviations

HPVv:

Human papillomavirus vaccine

HPV:

Human papillomavirus

ASIA:

Autoimmune/inflammatory syndrome induced by adjuvants

PC:

Phosphatidylcholine

FST:

Forced swimming test

MANOVA:

Multivariate analysis of variance

CNS:

Central nervous system

CFS:

Chronic fatigue syndrome

PS:

Phosphatidylserine

GC:

Glucocorticoid

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Acknowledgments

This work was supported by Judy and Stewart Colton as part of the Ph.D. Project of María-Teresa Arango.

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    Authors and Affiliations

    1. Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, 52621, Tel-Hashomer, Israel

      Shaye Kivity, Maria-Teresa Arango, Miri Blank & Yehuda Shoenfeld

    2. Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

      Shaye Kivity, Maria-Teresa Arango, Miri Blank & Yehuda Shoenfeld

    3. Rheumatic Disease Unit, Sheba Medical Center, Tel-Hashomer, Israel

      Shaye Kivity

    4. The Dr. Pinchas Borenstein Talpiot Medical Leadership Program 2013, Sheba Medical Center, Tel-Hashomer, Israel

      Shaye Kivity

    5. Doctoral Program in Biomedical Sciences, Universidad del Rosario, Bogotá, Colombia

      Maria-Teresa Arango

    6. Center for Autoimmune Diseases Research (CREA)-Statistics, School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Colombia

      Nicolás Molano-González

    7. Incumbent of the Laura Schwarz-Kip Chair for Research of Autoimmune Diseases, Tel Aviv University, Tel Aviv, Israel

      Yehuda Shoenfeld

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    1. Shaye Kivity
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    Correspondence to Yehuda Shoenfeld.

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    Yehuda Shoenfeld has acted as a consultant for the no-fault U.S. National Vaccine Injury Compensation Program. The other co-authors declare no competing interests.

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    Shaye Kivity and Maria-Teresa Arango have contributed equally to this work.

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    Kivity, S., Arango, MT., Molano-González, N. et al. Phospholipid supplementation can attenuate vaccine-induced depressive-like behavior in mice. Immunol Res 65, 99–105 (2017). https://doi.org/10.1007/s12026-016-8818-6

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    Keywords

    • Gardasil
    • Aluminum
    • Depression
    • Behavior
    • Autoimmunity