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Novel neutralising antibodies targeting the N-terminal helical region of the transmembrane envelope protein p15E of the porcine endogenous retrovirus (PERV)

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Abstract

Previously, immunising different species with the transmembrane envelope protein p15E of the porcine endogenous retrovirus (PERV), neutralising antibodies were induced which recognised epitopes in the fusion peptide proximal region (FPPR) and in the membrane-proximal external region (MPER). Only the MPER-specific antibodies were shown to neutralise and these antibodies targeted epitopes in the MPER similarly localised as the epitopes recognised by antibodies broadly neutralising HIV-1 such as 2F5 and 4E10. To study whether neutralising antibodies could be induced immunising with subunits of p15E, recombinant proteins corresponding to the N-terminal, the C-terminal helical region (NHR, CHR) and a p15E with a mutation in the Cys–Cys loop were produced. Whereas none of these antigens induced MPER-specific neutralising antibodies, the animals immunised with the FPPR/NHR subunit and the mutated p15E produced neutralising antibodies binding to the NHR. Therefore, for the first time, antibodies specific for the NHR and neutralising PERV were described.

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Acknowledgments

We thank M. Keller, D. Wirths, K. Braunmüller, and C.-M. Schmidt for excellent technical assistance, J. Kreutzberger for assistance in AktaExplorer purification and Daniela Neubert, Heide-Marie Lochotzke und Monika Hellwig, Federal Institute for Risk Assessment, Berlin, Germany, for their support of the animal experiments. The project was supported by a scholarship from the Hans Boeckler Foundation.

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The authors declare no conflicts of interest.

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  1. Centre for HIV and Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353, Berlin, Germany

    Alexander Waechter & Joachim Denner

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  1. Alexander Waechter
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  2. Joachim Denner
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Correspondence to Joachim Denner.

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Waechter, A., Denner, J. Novel neutralising antibodies targeting the N-terminal helical region of the transmembrane envelope protein p15E of the porcine endogenous retrovirus (PERV). Immunol Res 58, 9–19 (2014). https://doi.org/10.1007/s12026-013-8430-y

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