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Transcriptional target-based expression cloning of immunoregulatory molecules

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Abstract

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.

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Acknowledgments

The work referred to was supported by RSG-06-172-01-LIB from the American Cancer Society, PO1AI072677 and RO1AI078980 from the NIH, and funds from the Johns Hopkins University Institute for Cell Engineering. R.L. is supported by Ruth L. Kirschstein National Research Service Award F31AG031689. S.M.L. is supported by a Dr. Richard and Mavis Fowler and The Foundation for Advanced Research in the Medical Sciences, Inc. (FARMS) Fellowship. J.L.P is a recipient of a Kimmel Scholar Award from the Sidney Kimmel Foundation for Cancer Research and a Rita Allen Foundation Scholar.

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Authors and Affiliations

  1. Department of Biological Chemistry, Institute for Cell Engineering, and Graduate Program in Immunology, The Johns Hopkins University School of Medicine, 733 N. Broadway, BRB 607, Baltimore, MD, 21205, USA

    Rebecca L. Lamason, Stefanie M. Lew & Joel L. Pomerantz

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  1. Rebecca L. Lamason
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  2. Stefanie M. Lew
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  3. Joel L. Pomerantz
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Correspondence to Joel L. Pomerantz.

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Lamason, R.L., Lew, S.M. & Pomerantz, J.L. Transcriptional target-based expression cloning of immunoregulatory molecules. Immunol Res 47, 172–178 (2010). https://doi.org/10.1007/s12026-009-8148-z

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  • DOI: https://doi.org/10.1007/s12026-009-8148-z

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