Abstract
Alternate light sources such as ultraviolet, narrow band, and infrared have been used in an attempt to reveal the presence of bruising that is not otherwise apparent (inapparent). The following study evaluates the ability of alternate light sources to enhance visibility of bruises by employing an objective assessment of digital photography images in conjunction with histology. A pigskin model was employed with bruises created by injection of blood to be not visible or barely visible (inapparent) under white light. The pigskin was photographed using alternate light source illumination. Images were assessed using the program Fiji® to measure enhancement in terms of bruise length (cm). Photography results were compared with histology to confirm the presence of bruising. Violet and blue light sources produced the greatest enhancement, both with a p < 0.0001. Regions that were not bruises were also enhanced with light sources in this study, indicating that light sources are not specific, and that their use to enhance the visibility of bruising should be undertaken with caution.
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References
Langlois NE. The science behind the quest to determine the age of bruises—a review of the English language literature. Forensic Sci Med Pathol. 2007;3:241–51.
Vanezis P. Interpreting bruises at necropsy. J Clin Pathol. 2001;54:348–55.
Cluroe AD. Superficial soft-tissue injury. Am J Forensic Med Pathol. 1995;16:142–6.
Wright FD, Golden GS. The use of full spectrum digital photography for evidence collection and preservation in cases involving forensic odontology. Forensic Sci Int. 2010;201:59–67.
Lee W, Khoo B. Forensic light sources for detection of biological evidences in crime scene investigation: a review. Malays J Forensic Sci. 2010;1:17–28.
Holbrook DS, Jackson MC. Use of an alternate light source to assess strangulation victims. J Forensic Nurs. 2013;9:140–5.
Lombardi M, Canter J, Patrick PA, Altman R. Is fluorescence under an alternate light source sufficient to accurately diagnose subclinical bruising? J Forensic Sci. 2015;60:444–9.
Robin S, Courderot-Masuyer C, Tauzin H, et al. Use of a model of a blood-induced bruise for the evaluation of formulations on bruising. J Cosmet Dermatol Sci Appl. 2015;5:7.
Ross C, Byard RW, Langlois NE. Does the intensity of the inflammatory reaction in a bruise depend on its proximity to the site of trauma? Forensic Sci Med Pathol. 2013;9:358–62.
Marin N, Buszka J, Miller LS. Alternate light source imaging: forensic photography techniques. London: Routledge; 2013.
Hughes V, Ellis P, Burt T, Langlois N. The practical application of reflectance spectrophotometry for the demonstration of haemoglobin and its degradation in bruises. J Clin Pathol. 2004;57:355–9.
Akuwudike AR, Chikezie PC, Chilaka FC. Absorption spectra of normal adults and patients with sickle cell anaemia treated with hydrogen peroxide at two pH values. IJBC. 2013;5:129–35.
Sullivan TP, Eaglstein WH, Davis SC, Mertz P. The pig as a model for human wound healing. Wound Repair Regen. 2001;9:66–76.
Avon SL, Wood RE. Porcine skin as an in vivo model for ageing of human bite marks. J Forensic Odontostomatol. 2005;23:30–9.
Grosenbaugh DA, Alben JO, Muir WW. Absorbance spectra of inter-species hemoglobins in the visible and near infrared regions. J Vet Emerg Crit Care. 1997;7:36–42.
Brauers J, Helling S, Aach T. Multispectral image acquisition with flash light sources. J Imaging Sci Technol. 2009;53:31103-1.
Bernstein M, Nichols G, Blair J. The use of black and white infrared photography for recording blunt force injury. Clin Anat. 2013;26:339–46.
Rowan P, Hill M, Gresham G, Goodall E, Moore T. The use of infrared aided photography in identification of sites of bruises after evidence of the bruise is absent to the naked eye. J Forensic Leg Med. 2010;17:293–7.
Eastman Kodak Publication. Medical infrared photography. Publication No. N-1. New York: Eastman Kodak Co; 1973.
Tetley C, Young S. Digital infrared and ultraviolet imaging part 1: infrared. J Vis Commun Med. 2007;30:162–71.
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We would like to acknowledge the University of Adelaide Histology Services and funding provided by a Forensic Science SA summer scholarship.
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Olds, K., Byard, R.W., Winskog, C. et al. Validation of ultraviolet, infrared, and narrow band light alternate light sources for detection of bruises in a pigskin model. Forensic Sci Med Pathol 12, 435–443 (2016). https://doi.org/10.1007/s12024-016-9813-x
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DOI: https://doi.org/10.1007/s12024-016-9813-x