The transcription factor Yin Yang 1 (YY1) has an important regulatory role in tumorigenesis, but its implication in thyroid cancer has not been yet investigated. In the present study, we have analyzed the expression of YY1 in differentiated thyroid cancer and assessed the association of YY1 expression with clinical features. Expression of YY1 was evaluated in human thyroid cancer cell lines, a series of matched normal/tumor thyroid tissues and in a thyroid cancer tissue microarray, using real-time PCR, Western blot, and/or immunohistochemistry. YY1 was overexpressed in thyroid cancer cells, at transcription and protein levels. A significant increase of YY1 mRNA was also observed in tumor thyroid tissues. Moreover, immunohistochemical analysis of the thyroid cancer tissue microarray revealed that both papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC) present increased YY1 protein levels (48 and 19 %, respectively). After stratification by the level of YY1 protein, positive YY1 expression identifies 88 % of patients with PTC. The association of YY1 expression with clinicopathological features in PTC and FTC showed that YY1 expression was related with age at diagnosis. Our data indicates for the first time overexpression of YY1 in differentiated thyroid cancer, with YY1 being more frequently overexpressed in the PTC subtype.
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We thank all the subjects participating in this study and Cristian Valiente for his technical support. This work was partially funded by the Spanish Ministry of Education and Science (project SAF2007-6338) and the Generalitat de Catalunya (CIRIT; 2009SGR-725). J. Arribas was supported by a predoctoral fellowship (PIF) from the Universitat Autònoma de Barcelona.
Conflict of Interest
The authors declare that they have no conflict of interest.
Patient’s data were obtained and handled in accordance with the ethical standards of the Human Ethics Committee of the Hospital (Comitè Ètic d’Investigació Clínica, CEIC, Hospital Universitari Vall d’Hebron) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Arribas, J., Castellví, J., Marcos, R. et al. Expression of YY1 in Differentiated Thyroid Cancer. Endocr Pathol 26, 111–118 (2015). https://doi.org/10.1007/s12022-015-9359-6
- Thyroid cancer
- Differentiated thyroid cancer
- YY1 expression
- Transcription factor Yin Yang 1