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PTTG is a Secretory Protein in Human Pituitary Adenomas and in Mouse Pituitary Tumor Cell Lines

Endocrine Pathology volume 18, Article number: 8 (2007) Cite this article

Abstract

The pituitary tumor-transforming gene (PTTG) is a homolog of yeast Securin, which arrests the activation of Separin to induce sister chromatid separation in the transition from metaphase to anaphase. Pituitary tumor-transforming gene is also known to induce angiogenesis during pituitary tumorigenesis. It has not been clarified whether PTTG functions as a cytoplasmic or a nuclear protein. Our immunohistochemical study indicated that PTTG is localized in the cytoplasm of pituitary tumor cells. In the present study, confocal laser scanning microscopy (CLSM) analysis of human pituitary adenomas and immunoelectron microscopy of the mouse pituitary cell line, AtT-20, demonstrated the localization of PTTG in the Golgi apparatus and vesicles. Secreted PTTG was detected by immunoblotting from culture medium of mouse pituitary tumor cell lines. Our results suggested that PTTG is a secretory protein produced by pituitary tumor cells. In addition, PTTG may exert autocrine and/or paracrine functions as a newly proposed important pathway for the action of PTTG.

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Affiliations

  1. Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa, 259-1193, Japan

    Takeo Minematsu, Noboru Egashira, Hanako Kajiya, Susumu Takekoshi & Robert Yoshiyuki Osamura

  2. Education and Research Support Center, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa, 259-1193, Japan

    Yoshiko Itoh, Hideo Tsukamoto & Johbu Itoh

  3. Department of Neurosurgery, Nippon Medical School, 1-1-5, Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan

    Mao Takei, Naoko Sanno & Akira Teramoto

Authors
  1. Takeo Minematsu
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  2. Noboru Egashira
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  3. Hanako Kajiya
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  4. Mao Takei
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  5. Susumu Takekoshi
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  6. Yoshiko Itoh
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  7. Hideo Tsukamoto
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  8. Johbu Itoh
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  9. Naoko Sanno
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  10. Akira Teramoto
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  11. Robert Yoshiyuki Osamura
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Correspondence to Robert Yoshiyuki Osamura.

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Minematsu, T., Egashira, N., Kajiya, H. et al. PTTG is a Secretory Protein in Human Pituitary Adenomas and in Mouse Pituitary Tumor Cell Lines. Endocr Pathol 18, 8 (2007). https://doi.org/10.1007/s12022-007-0005-9

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  • DOI: https://doi.org/10.1007/s12022-007-0005-9

Keywords

  • PTTG
  • secretion
  • pituitary
  • adenoma
  • tumorigenesis