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Intestinal Trefoil Factor 3: a new biological factor mediating gut-kidney crosstalk in diabetic kidney disease

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Abstract

Purpose

To investigate the effect of TFF3 in the pathogenesis of Diabetic Kidney Disease (DKD), and explore the dynamic changes of TFF3 expression pattern in renal injury process.

Methods

DKD animal model was established by streptozotocin (STZ) (40 mg/kg/d, ip, for 5 days, consecutively) combined with the high fat diet (HFD) for 12 weeks. While animals were sacrificed at different time stages in DKD process (4 weeks, 8 weeks and 12 weeks, respectively).

Results

STZ combined with high-fat diet induced weight gain, increased blood glucose and decreased glucose tolerance in DKD mice. Compared to the control group, the DKD group exhibits extracellular matrix (ECM) accumulation and the renal injury was aggravated in a time-dependent manner. The TFF3 expression level was decreased in kidney, and increased in colon tissue.

Conclusion

TFF3 is not only expressed in colon, but also expressed in renal medulla and cortex. TFF3 might be play a pivotal role in renal mucosal repair by gut-kidney crosstalk, and protect renal from high glucose microenvironment damage.

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Author contributions:

T.Z.: Writing, original draft and preparation. Y.H.Z.: Drawing and drafting; J.T.: Formal analysis, Software. X.L.R.: review, editing and Supervision. Y.Q.Y.: Writing, review & editing, Supervision, Conceptualization and Project administration.

Funding

This work was supported by Key Project of National Natural Science Foundation of China (82204704); Basic and Applied Basic Research Special Project, Guangzhou Science and Technology Bureau (SL2024A04J00581).

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Authors and Affiliations

  1. Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education; Guangdong TCM Key Laboratory for Metabolic Diseases, Guangzhou Higher Education Mega Center, Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Tao Zhang, Yinghui Zhang, Jie Tao, Xianglu Rong & Yiqi Yang

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  1. Tao Zhang
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Correspondence to Yiqi Yang.

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All animal experiments were approved by the Animal Experiment Ethics Committee of Guangdong Pharmaceutical University (gdpulac2019180).

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These authors contributed equally: Tao Zhang, Yinghui Zhang

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Zhang, T., Zhang, Y., Tao, J. et al. Intestinal Trefoil Factor 3: a new biological factor mediating gut-kidney crosstalk in diabetic kidney disease. Endocrine 84, 109–118 (2024). https://doi.org/10.1007/s12020-023-03559-5

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