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Expression of the angiogenesis markers endocan in Cushing’s syndrome due to cortisol producing adenoma

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Abstract

Purpose

In this study, we aimed to investigate the endocan expression in tissue samples of patients diagnosed with Cushing’s syndrome (CS) due to Cortisol-Producing-Adenoma (CPA) and compare it with normal adrenal cortex tissue, and also to evaluate the correlations of endocan expression with clinical and histopathological features.

Methods

11 patients who were operated for CS due to CPA between 2009–2021 and 14 control subjects with normal adrenal cortex were included in the study. Demographic, laboratory and clinicopathological data of the patients were recorded. Sections of 4–5 µm thickness were taken from paraffin blocks of patients diagnosed with CS due to CPA and control subjects with normal adrenal cortex tissue. The sections were then stained in a closed system automatic immunohistochemical staining device to perform immunohistochemical analysis. The endocan positive grade were determined based on the proportion of stained cells on a scale of negative to strong.

Results

The number of subjects with positive endocan expression and the mean endocan expression level in the CS group were significantly higher than the control group (p = 0.005, p < 0.001, respectively). No correlation was found between endocan expression and clinical and histopathological features.

Conclusion

According to the results of our study, endocan overexpression in CPA tissues may be related to the hormonal functionality of CPA.

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Author information

Authors and Affiliations

  1. Department of Internal Medicine, Division of Endocrinology and Metabolism, Muş State Hospital, Muş, Turkey

    Mustafa Can

  2. Department of Internal Medicine, Division of Endocrinology and Metabolism, Tokat State Hospital, Tokat, Turkey

    Muhammet Kocabaş

  3. Department of Internal Medicine, Division of Endocrinology and Metabolism, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey

    Melia Karaköse, Mustafa Kulaksızoğlu & Feridun Karakurt

  4. Department of Pathology, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey

    Hacı Hasan Esen

Authors
  1. Mustafa Can
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  2. Muhammet Kocabaş
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  3. Melia Karaköse
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  4. Hacı Hasan Esen
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  5. Mustafa Kulaksızoğlu
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  6. Feridun Karakurt
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by M.C., M.K., M.K., and H.H.E. The first draft of the manuscript was written by M.K., F.K., and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Mustafa Can.

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Conflict of interest

The authors declare no competing interests.

Ethics approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of University Necmettin Erbakan (22.05.2020/No: 2020/2504).

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Can, M., Kocabaş, M., Karaköse, M. et al. Expression of the angiogenesis markers endocan in Cushing’s syndrome due to cortisol producing adenoma. Endocrine 79, 554–558 (2023). https://doi.org/10.1007/s12020-022-03250-1

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  • DOI: https://doi.org/10.1007/s12020-022-03250-1

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