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Expression profile of serum-related exosomal miRNAs from parathyroid tumor

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Abstract

Objective

The expression pattern of exosomal miRNAs derived from parathyroid tumor is still unknown. In the present work, we aimed to examine the differences on microRNA (miRNA) expression, present in serum exosomes, by comparing parathyroid carcinoma (PC) and parathyroid adenoma (PA).

Methods

MiRNA expression profile of serum exosomes, derived from 4 PC patients and 4 PA patients, were analyzed by next-generation sequencing technology. The differential expressions of target miRNAs were further verified in both serum exosomes and tissues of PC/PA patients by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Lastly, receiver operating characteristic (ROC) curves were plotted to investigate the efficiency of target exosomal miRNAs in distinguishing PC patients from PA controls.

Results

Multiple differentially expressed miRNAs of serum exosomes were screened out by sequencing. Based on this screening, hsa-miR-146b-5p (p = 0.0846), hsa-miR-27a-5p (p = 0.0412), hsa-miR-93-5p (p = 0.73), hsa-miR-381-3p (p = 0.1239) and hsa-miR-134-5p (p = 0.0694) were upregulated in the serum exosomes of PC patients. These results were validated by qPCR, where the trend on differential miRNA expression was consistent with the sequencing results. Specifically, the expression of exosomal hsa-miR-27a-5p was able to clearly distinguish PC patients from PA controls, and related analysis indicated that the area under the ROC curve was 0.8594 (p = 0.0157).

Conclusions

Here we present, for the first time, the miRNA expression profile of serum exosomes derived from PC patients. Based on this result, we presently suggest that the exosomal hsa-miR-27a-5p may serve as a putative tumor marker for preoperative identification of PC and PA subjects.

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Data availability

All data generated or analyzed during this study are included in this published article.

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Acknowledgements

We appreciated Thyroid and Neck Surgery of Beijing Chao-Yang Hospital for providing start-up funding for this study.

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Authors and Affiliations

  1. Department of Thyroid and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, 100020, Beijing, China

    Jiacheng Wang, Qian Wang, Teng Zhao, Xing Liu, Ge Bai, Yunhui Xin, Hong Shen & Bojun Wei

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  1. Jiacheng Wang
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  2. Qian Wang
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  3. Teng Zhao
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  4. Xing Liu
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  5. Ge Bai
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  6. Yunhui Xin
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  7. Hong Shen
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  8. Bojun Wei
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Contributions

J.W. analyzed and interpreted the experimental data, and was a major contributor in writing the manuscript. All authors read and approved the final manuscript

Corresponding author

Correspondence to Bojun Wei.

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The authors declare that they have no conflict of interest.

Ethics approval

This study was approved by the Ethics Review Board of Beijing Chao-Yang Hospital, Capital Medical University.

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All patients have signed a written informed consent to participate in this work.

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Cite this article

Wang, J., Wang, Q., Zhao, T. et al. Expression profile of serum-related exosomal miRNAs from parathyroid tumor. Endocrine 72, 239–248 (2021). https://doi.org/10.1007/s12020-020-02535-7

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  • DOI: https://doi.org/10.1007/s12020-020-02535-7

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