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Association of PPARγ gene expression with postprandial hypertriglyceridaemia and risk of type 2 diabetes mellitus

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Abstract

Purpose

Peroxisome proliferator-activated receptor γ (PPARγ) gene is strongly associated with type 2 diabetes mellitus, as well as postprandial lipemia, and plays an important role in Wnt dependent adipogenesis in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT). We aimed to study the expression of PPARγ gene in SAT and VAT to find out its correlation with postprandial hypertriglyceredemia and glucose intolerance.

Methods

Thirty subjects who were scheduled to undergo abdominal surgery were recruited in three groups (n = 10 in NGT, n = 10 in prediabetes, and n = 10 in T2DM). A standardized oral fat challenge was performed. Anthropometry, plasma glucose, HbA1c, and fasting serum insulin were also measured. SAT and VATs were collected during surgery for PPARγ gene expression studies by real-time PCR.

Results

PPARγ gene expression was 5.5-fold lower in T2DM and 1.7-fold lower in prediabetes as compared with NGT subjects in VAT. There was a significant negative correlation of expression of PPARγ gene in VAT {Tgauc (r = −0.57, p < 0.007), Peak Tg (r = −0.51, p < 0.01)} as well as in subcutaneous adipose tissue {Tgauc (r = −0.45, p < 0.02)} with PPTg responses measures.

Conclusion

Reduced adipocyte expression of PPARγ gene and the resultant postprandial hypertriglyceredemia is associated with greater risk of diabetes and prediabetes.

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Data availability

Data will be available on reasonable request.

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Acknowledgements

This work was supported by Indian Council of Medical Research, New Delhi (Grant No. 5/4/5-3/Diab.-16-NCD-II).

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Authors and Affiliations

  1. Department of Endocrinology, University College of Medical Sciences, University of Delhi, Delhi, India

    B. K. Mishra & S. V. Madhu

  2. Department of Biochemistry, University College of Medical Sciences, University of Delhi, Delhi, India

    B. D. Banerjee

  3. Department of Surgery, University College of Medical Sciences, University of Delhi, Delhi, India

    V. Agrawal

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  1. B. K. Mishra
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Correspondence to S. V. Madhu.

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Prior Presentation Parts of this study were presented in abstract form at the RSSDI DC 14th Annual Conference 2018, 21st October 2018 at Hotel the Lalit, New Delhi.

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Mishra, B.K., Banerjee, B.D., Agrawal, . et al. Association of PPARγ gene expression with postprandial hypertriglyceridaemia and risk of type 2 diabetes mellitus. Endocrine 68, 549–556 (2020). https://doi.org/10.1007/s12020-020-02257-w

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