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Screening and functional studies of long noncoding RNA in subjects with prediabetes

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Abstract

Background

In recent years, long noncoding RNAs (LncRNAs) have been found to play an important role in type 2 diabetes mellitus. However, research on the relationship between LncRNAs and prediabetes is still emerging.

Objectives

The study aim was to screen differently expressed LncRNAs and understand their localization and function in patients with prediabetes.

Methods

We used microarray analysis to screen LncRNAs in prediabetes participants.To further clarify the localization and function of the expressed mRNAs, we used gene ontology analysis and pathway analysis. Then, internal validations were performed using individual quantitative real-time polymerase chain reaction (qRT-PCR) assays.

Results

We identified 55 differently expressed LncRNAs and 36 mRNAs in prediabetes participants comparing with controls. Gene ontology analysis indicated that the most enriched transcript terms were multicellular organismal process, plasma membrane, and binding. Pathway analysis indicated that the differently expressed mRNAs were involved in processes such as starch and sucrose metabolism, pantothenate and coenzyme A biosynthesis, and nicotinate and nicotinamide metabolism. The qRT-PCR results showed a trend consistent with the microarray results in 30 patients and 30 healthy controls.

Conclusions

We found aberrantly expressed LncRNAs and mRNAs in prediabetes subjects, and demonstrated that these LncRNAs are involved in the entire prediabetes biological process.

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Acknowledgements

We thank Diane Williams, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

Funding

This work was supported by a grant from the Preventive Medicine research projects of Jiangsu Province Health Department in 2018 (Y2018016) and the Medical Youth Talent of the Project of Invigorating Health Care through Science in Jiangsu Province (QNRC2016375), the Preventive Medicine Research Projects of Jiangsu Province Health Department in 2015 (Y2015010), and the Science and Technology projects of Xuzhou City in 2015 (KC15SM046). The researchers had no relationships with the funder. The study funding had no influence on the study design, data collection, analysis, interpretation of data, writing of the report, or decision to submit the article for publication.

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Authors and Affiliations

  1. Department of Control and Prevention of Chronic Non-Communicable Diseases, Xuzhou Center for Disease Control and Prevention, Xuzhou, Jiangsu, China

    Pan Zhang, Xuan Zhu, Yangguang Du, Zongmei Dong, Cheng Qiao, Ting Li, Peipei Chen & Peian Lou

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  1. Pan Zhang
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  2. Xuan Zhu
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  3. Yangguang Du
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  4. Zongmei Dong
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  7. Peipei Chen
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  8. Peian Lou
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Contributions

PZ designed the study, acquired and analyzed data, and drafted and reviewed the manuscript. XZ and YD conducted experiments, reviewed the manuscript, and contributed to the method. ZD conceived and investigated the study, analyzed data, and reviewed the manuscript. CQ and TL researched data, contributed to the discussion, and reviewed the manuscript. PC analyzed data and contributed to the introduction. PL conceived and designed the study, acquired and analyzed data, drafted and reviewed the manuscript, obtained funding, provided administrative support, and supervised the study. PL is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis, reviewed and edited the manuscript.

Corresponding author

Correspondence to Peian Lou.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study was conducted with approval from the ethics committee of Xuzhou Center for Disease Control and Prevention. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Zhang, P., Zhu, X., Du, Y. et al. Screening and functional studies of long noncoding RNA in subjects with prediabetes. Endocrine 68, 296–305 (2020). https://doi.org/10.1007/s12020-020-02226-3

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  • DOI: https://doi.org/10.1007/s12020-020-02226-3

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