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Association of cord blood des-acyl ghrelin with birth weight, and placental GHS-R1 receptor expression in SGA, AGA, and LGA newborns

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Abstract

Although ghrelin in cord blood has been associated to birth weight, its role in fetal and postnatal growth has not been elucidated. The aim of this study was to analyze total ghrelin, acyl ghrelin (AG), and des-acyl ghrelin (DAG) in cord blood of newborns with idiopathic birth weight alterations, and to evaluate protein expression of placental GHS-R1, in order to investigate their correlation with birth weight and placental weight. We performed a cross-sectional comparative study in umbilical cord blood and placentas from healthy mothers of SGA, AGA, and LGA (small, adequate and large for gestational age) term newborns (n = 20 per group). Cord blood total ghrelin, AG, and DAG were measured by ELISA, and placental GHS-R1 expression was evaluated by Western blot. Cord blood DAG was higher in SGA compared to AGA newborns (902.1 ± 109.1 and 597.4 ± 58.2 pg/ml, respectively, p = 0.01) while LGA and AGA showed similar values (627.2 ± 76.4 pg/ml for LGA, p = 0.80). DAG negatively correlated with birthweight (r = −0.31, p = 0.02) and placental weight (r = −0.33, p = 0.02). No differences in AG or total ghrelin were found. GHS-R1 protein in placenta was not differentially expressed among SGA, AGA, and LGA. Our results suggest a role of DAG in intrauterine growth. Further studies are needed in order to elucidate the mechanisms by which DAG participates in fetal growth.

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Acknowledgments

This work was supported by research funds from University of Guanajuato (0095/13) and FOMIX Consejo Nacional de Ciencia y Tecnología (CONACYT)—Guanajuato State Government (GTO-2012-C03-195238). MLLVM was recipient of a postdoctoral Fellowship Scholarship from CONACYT (CVU: 217876). MIGD was recipient of a PhD CONACYT Scholarship (CVU: 217761). We are indebted to Dr. Cutberto Torres Torres, Dr. Gabriel Corona Martínez, and Dr. Olimpia Azpeitia for their invaluable support.

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Authors and Affiliations

  1. Medical Sciences Department, Health Sciences Division, University of Guanajuato, Leon Campus. 20 de Enero No. 929 Col. Obregon, 37320, Leon, Guanajuato, Mexico

    Martha I. González-Domínguez, Maria-Luisa Lazo-de-la-Vega-Monroy, Silvio Zaina, Juan Manuel Malacara & Gloria Barbosa-Sabanero

  2. Biology Department, Natural and Exact Sciences Division, University of Guanajuato, Guanajuato Campus, Guanajuato, Mexico

    Myrna Sabanero

  3. UMAE No. 48 IMSS, Leon, Guanajuato, Mexico

    Leonel Daza-Benítez

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  1. Martha I. González-Domínguez
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  2. Maria-Luisa Lazo-de-la-Vega-Monroy
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  3. Silvio Zaina
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  5. Leonel Daza-Benítez
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  6. Juan Manuel Malacara
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  7. Gloria Barbosa-Sabanero
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Correspondence to Gloria Barbosa-Sabanero.

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This study was approved by the corresponding ethics committees, written informed consent was obtained from participants, and all procedures were performed according to the Mexican General Health Laws and the Declaration of Helsinki.

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Informed consent was obtained from all individual participants included in the study.

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González-Domínguez, M.I., Lazo-de-la-Vega-Monroy, ML., Zaina, S. et al. Association of cord blood des-acyl ghrelin with birth weight, and placental GHS-R1 receptor expression in SGA, AGA, and LGA newborns. Endocrine 53, 182–191 (2016). https://doi.org/10.1007/s12020-015-0833-1

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  • DOI: https://doi.org/10.1007/s12020-015-0833-1

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