Long-term exposure to electromagnetic radiation from mobile phones and Wi-Fi devices decreases plasma prolactin, progesterone, and estrogen levels but increases uterine oxidative stress in pregnant rats and their offspring
We investigated the effects of mobile phone (900 and 1800 MHz)- and Wi-Fi (2450 MHz)-induced electromagnetic radiation (EMR) exposure on uterine oxidative stress and plasma hormone levels in pregnant rats and their offspring. Thirty-two rats and their forty newborn offspring were divided into the following four groups according to the type of EMR exposure they were subjected to: the control, 900, 1800, and 2450 MHz groups. Each experimental group was exposed to EMR for 60 min/day during the pregnancy and growth periods. The pregnant rats were allowed to stand for four generations (total 52 weeks) before, plasma and uterine samples were obtained. During the 4th, 5th, and 6th weeks of the experiment, plasma and uterine samples were also obtained from the developing rats. Although uterine lipid peroxidation increased in the EMR groups, uterine glutathione peroxidase activity (4th and 5th weeks) and plasma prolactin levels (6th week) in developing rats decreased in these groups. In the maternal rats, the plasma prolactin, estrogen, and progesterone levels decreased in the EMR groups, while the plasma total oxidant status, and body temperatures increased. There were no changes in the levels of reduced glutathione, total antioxidants, or vitamins A, C, and E in the uterine and plasma samples of maternal rats. In conclusion, although EMR exposure decreased the prolactin, estrogen, and progesterone levels in the plasma of maternal rats and their offspring, EMR-induced oxidative stress in the uteri of maternal rats increased during the development of offspring. Mobile phone- and Wi-Fi-induced EMR may be one cause of increased oxidative uterine injury in growing rats and decreased hormone levels in maternal rats.
KeywordsAntioxidants Uterine Hormone Growing rat Electromagnetic radiation
Extremely low electrical field
Enzyme-linked immunosorbent assay
Nicotinamide adenine dinucleotide phosphate
Reactive oxygen species
Total antioxidant status
Total oxidant status
Wireless local area network
The Scientific Research Unit (BAP) of the Suleyman Demirel University (Project Number: 3536-TU2-13) supported this study. The authors are grateful to Muhammet Şahin (Department of Biophysics) for helping with the antioxidant and oxidant analyses. In addition, the authors wish to thank Assoc. Prof. Dr. Selçuk Çömlekçi (Electronics and Communication Engineering, Suleyman Demirel University, Isparta, Turkey), for calculation of the specific absorption rates.
Mustafa Nazıroğlu formulated the hypothesis for the present study and wrote the report. Murat Yüksel was involved in facilitating the EMR exposure procedures as well as in managing the experimental rats. Mehmet Okan Özkaya made critical revisions to the manuscript.
Compliance with Ethical Standards
Conflict of Interest
The authors declare no conflicts of interest.
- 9.F. Poulletier de Gannes, B. Billaudel, E. Haro et al., Rat fertility and embryo fetal development: influence of exposure to the Wi-Fi signal. Reprod. Toxicol. 36C, 1–5 (2012)Google Scholar
- 14.A. Özorak, M. Nazıroğlu, Ö. Çelik, M. Yüksel, D. Özçelik, M.O. Özkaya, H. Çetin, M.C. Kahya, S.A. Kose, Wi-Fi (2.45 GHz) and mobile phone (900 and 1800 MHz)-induced risks on oxidative stress and elements in kidney and testis of rats during pregnancy and the development of newborns. Biol. Trace Elem. Res. 156(1-3), 221–229 (2013)CrossRefPubMedGoogle Scholar
- 23.J. Suzuki, N. Katoh, A simple and cheap method for measuring vitamin A in cattle using only a spectrophotometer. Jpn. J. Vet. Sci. 52, 1282–1284 (1990)Google Scholar
- 27.M.Y. Obolenskaya, N.M. Teplyuk, R.L. Divi, M.C. Poirier, N.B. Filimonova, M. Zadrozna, M.J. Pasanen, Human placental glutathione S-transferase activity and polycyclic aromatic hydrocarbon DNA adducts as biomarkers for environmental oxidative stress in placentas from pregnant women living in radioactivity- and chemically-polluted regions. Toxicol. Lett. 196(2), 80–86 (2010)CrossRefPubMedPubMedCentralGoogle Scholar
- 30.I. Belyaev, Non-thermal biological effects of microwaves. Microw. Rev. 11, 13–29 (2005)Google Scholar
- 34.H. Çetin, M. Nazıroğlu, Ö. Çelik, M. Yüksel, N. Pastacı, M.O. Özkaya, Liver antioxidant stores protect the brain from electromagnetic radiation (900 and 1800 MHz)-induced oxidative stress in rats during pregnancy and the development of offspring. J. Matern. Fetal. Neonatal. Med. 27, 1915–1921 (2014)CrossRefPubMedGoogle Scholar