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γδ Τ cells enhance B cells for antibody production in Hashimoto’s thyroiditis, and retinoic acid induces apoptosis of the γδ Τ cell

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Abstract

TCR γδ+ Τ cells are important in the pathogenesis of inflammatory and autoimmune conditions. This study investigated the effect of γδ T cells on autoantibody production in patients with Hashimoto’s thyroiditis (HT). A total of 148 subjects were enrolled, including 99 patients with HT, 5 with simple goiters, and 44 healthy controls. Peripheral blood and thyroid mononuclear cells were subjected to flow cytometric analysis. Thyroid tissues underwent immunofluorescent staining and immunohistochemistry for γδ T cells and anti-thyroid antibody detection. Antibody production was measured by ELISA and automated chemiluminescent immunoassays. And activation and apoptosis of peripheral blood γδT cells and B cells were measured by flow cytometric analysis. The percentage of γδ T cells were greater in thyroid tissue from HT patients than that of goiter patients (n = 5, 5.33 ± 1.20 vs. 2.07 ± 0.44 %; P < 0.05), with the Vδ1+ γδ T cell subset especially dominant. Frequencies of CD69 (8.42 ± 1.08 vs. 1.60 ± 0.38 %, P < 0.001), HLA-DR (58.12 ± 6.36 vs. 37.82 ± 3.70 %, P < 0.05), CD40L (1.58 ± 0.35 vs. 0.15 ± 0.05 %, P < 0.01), and ICOS (2.78 ± 0.66 vs. 0.28 ± 0.13 %, P < 0.01) expressed on γδ T cells from HT patients (n = 19) were significantly increased compared with those of healthy controls (n = 15). More importantly, γδ T cells from HT patients enhanced B cells for antibody production, and all-trans retinoic acid (ATRA) treatment inhibited the effect by inducing apoptosis of γδ Τ cells. γδ Τ cells appear to play an important role in the pathogenesis of HT, and ATRA might be an effective regulator for HT patients.

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Acknowledgments

This work was supported by Grants from the National Natural Science Foundation of China (81370889, 81270801) and the Natural Science Foundation of Jiangsu Province (BK20131248, BK20131245).

Conflict of interest

The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang, 212001, China

    Hongli Liu, Tingting Zheng, Chengcheng Xu, Fei Wu, Ling Bu, Xiao Mou, Yuepeng Zhou, Guoyue Yuan, Deyu Chen & Chaoming Mao

  2. Institute of Oncology, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang, 212001, China

    Hongli Liu, Tingting Zheng, Chengcheng Xu, Fei Wu, Ling Bu, Xiao Mou, Yuepeng Zhou, Deyu Chen & Chaoming Mao

  3. Department of Laboratory Medicine, Nantong Tumor Hospital, Nantong, 226361, China

    Hongli Liu

  4. Department of Laboratory Immunology, Jiangsu University School of Medicine, Zhenjiang, 212001, China

    Yufei Mao & Shengjun Wang

  5. Department of Pediatrics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China

    Tong Zhou

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  1. Hongli Liu
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Liu, H., Zheng, T., Mao, Y. et al. γδ Τ cells enhance B cells for antibody production in Hashimoto’s thyroiditis, and retinoic acid induces apoptosis of the γδ Τ cell. Endocrine 51, 113–122 (2016). https://doi.org/10.1007/s12020-015-0631-9

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  • DOI: https://doi.org/10.1007/s12020-015-0631-9

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