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Protective effect of Schisandrae chinensis oil on pancreatic β-cells in diabetic rats

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Abstract

Islet cell dysfunction in type 2 diabetes is primarily attributed to increased apoptosis of pancreatic β-Cells. The aim of the present study was to investigate the effects of Schisandrae chinensis oil on pancreatic β-Cells in type 2 diabetes mellitus rats and the associated molecular mechanisms of action. Wistar rats were randomly divided into diabetic rats and control rats, diabetic rats treated with Schisandrae chinensis oil (1 mg/kg), and control rats treated with Schisandrae chinensis oil. The serum fasting blood glucose, insulin, total cholesterol, and triglyceride levels along with MDA content, SOD and CAT activities in pancreatic tissues were measured. TUNEL was used to observe the apoptosis of rat pancreatic cells. Western blot was used to determine specific protein expression. The results showed that the oil significantly decreased fasting blood glucose, total cholesterol, triglyceride levels as well as the pancreatic MDA, but increased SOD and CAT activities. The protein expression of Bcl-2, PDX-1, GLUT-2, and GCK but not caspase 3 was significantly enhanced in the oil-treated rats compared with diabetic rats. However, Bax content was not significantly different between the control and DM groups. Schisandra chinensis oil improves pancreatic β-cell function by enhancing antioxidant potential of the pancreas, upregulating the expression of anti-apoptotic genes, increasing expression of glucose metabolism, and delaying islet cell apoptosis.

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Acknowledgments

This work was funded by project “2013-199” supported by the Education Department of Jilin Province, project “201262503” supported by Sci-tech Department of Jilin City and project “20122082” the Healthe Department of Jilin Province.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Pharmacy, Beihua University, Jilin, 132013, Jilin, People’s Republic of China

    Liping An, Chunmei Wang, Xiao Han, Guangyu Xu, Guangxin Yuan, Hongyu Li, Yu Sheng, Manli Wang, Jingbo Sun, Jinzhuo Zhan, Hui Sun, Na Li & Peige Du

  2. Affiliated Hospital, Beihua University, Jilin, 132013, Jilin, People’s Republic of China

    Meizhen Fan & Fuxiang Ding

  3. Institute of Special Economic Plants and Wildlives Utilization, Chinese Academy of Agricultural Sciences, Jilin, 132019, People’s Republic of China

    Yingping Wang

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  1. Liping An
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Correspondence to Fuxiang Ding or Peige Du.

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An, L., Wang, Y., Wang, C. et al. Protective effect of Schisandrae chinensis oil on pancreatic β-cells in diabetic rats. Endocrine 48, 818–825 (2015). https://doi.org/10.1007/s12020-014-0375-y

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  • DOI: https://doi.org/10.1007/s12020-014-0375-y

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